HPLC with electrochemical and fluorescence detection procedures for the diagnosis of inborn errors of biogenic amines and pterins

• Published in: Journal of neuroscience methods Vol. 142; no. 1; pp. 153 - 158
• Main Authors: Ormazabal, Aida, García-Cazorla, Angels, Fernández, Yolanda, Fernández-Álvarez, Emilio, Campistol, Jaume, Artuch, Rafael
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.03.2005
• Subjects: Adolescent; Age Factors; Biogenic amines; Biogenic Amines - cerebrospinal fluid; Biopterins - cerebrospinal fluid; Brain Chemistry - genetics; Brain Diseases, Metabolic, Inborn - cerebrospinal fluid; Brain Diseases, Metabolic, Inborn - diagnosis; Brain Diseases, Metabolic, Inborn - physiopathology; Cerebrospinal fluid; Child; Child, Preschool; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Chromatography, High Pressure Liquid - standards; Electrochemistry - instrumentation; Electrochemistry - methods; Female; Fluorescent Dyes; Homovanillic Acid - cerebrospinal fluid; Humans; Hydroxyindoleacetic Acid - cerebrospinal fluid; Inborn errors of metabolism; Infant; Infant, Newborn; Male; Neopterin - cerebrospinal fluid; Paediatric patients; Predictive Value of Tests; Pterins; Pterins - cerebrospinal fluid; Reference Standards; Sample Size; Inborn errors of metabolism; Cerebrospinal fluid; Pterins; Paediatric patients; Biogenic amines

The analysis of biogenic amines (BA) and pterins in cerebrospinal fluid (CSF) is essential for the early diagnosis of neurotransmission defects in the paediatric age. Our aim was to standardize previously reported HPLC procedures for the analysis of BA and pterins in CSF and to establish reference v...