Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis
Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern p...
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Published in | Science (American Association for the Advancement of Science) Vol. 379; no. 6629; pp. 272 - 277 |
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Main Authors | , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
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United States
The American Association for the Advancement of Science
20.01.2023
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Abstract | Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the
Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals. |
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AbstractList | Parsing nodulation pathwaysLegumes benefit from symbiotic microbes resident in root nodules that fix nitrogen, whereas most other plants depend on externally supplied or gathered nitrogen. Rübsam et al. identified a bifunctional receptor complex in the model legume Lotus japonicus that initiates development and infection of the root nodules. Development of root nodules could be driven solely by the intracellular domains containing the kinases once the receptor complex was formed, but the Nod factor–recognizing ectodomains were required to support rhizobial infection. Receptors identified in the cereal barley, which does not form symbiotic nitrogen-fixing root nodules, were able to support Lotus root nodule organogenesis, suggesting that the path to nitrogen fixation in cereals might be shorter than we thought. —PJH Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals. Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals. Parsing nodulation pathways Legumes benefit from symbiotic microbes resident in root nodules that fix nitrogen, whereas most other plants depend on externally supplied or gathered nitrogen. Rübsam et al . identified a bifunctional receptor complex in the model legume Lotus japonicus that initiates development and infection of the root nodules. Development of root nodules could be driven solely by the intracellular domains containing the kinases once the receptor complex was formed, but the Nod factor–recognizing ectodomains were required to support rhizobial infection. Receptors identified in the cereal barley, which does not form symbiotic nitrogen-fixing root nodules, were able to support Lotus root nodule organogenesis, suggesting that the path to nitrogen fixation in cereals might be shorter than we thought. —PJH Llama nanobodies help identify the receptor complex that controls nodule organogenesis in plant symbiosis with nitrogen-fixing bacteria. |
Author | Radutoiu, Simona Ji, Hongtao Rübsam, Henriette Lironi, Damiano Abel, Nikolaj B Hansen, Simon B Madsen, Lene H Liu, Huijun de Jong, Noor Nadzieja, Marcin Andersen, Kasper R Krönauer, Christina Kolte, Marie V Abel, Dörte Stougaard, Jens |
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Snippet | Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach... Parsing nodulation pathwaysLegumes benefit from symbiotic microbes resident in root nodules that fix nitrogen, whereas most other plants depend on externally... |
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SubjectTerms | Cell Membrane - metabolism Cereals Gene Expression Regulation, Plant Infections Kinases Legumes Lipopolysaccharides - metabolism Lotus Medicago truncatula Nanobodies Nitrogen fixation Nitrogenation Nodulation Nodules Organogenesis Plant Proteins - genetics Plant Proteins - metabolism Receptors Root nodules Root Nodules, Plant - metabolism Signal Transduction Single-Domain Antibodies Symbiosis Symbiosis - physiology |
Title | Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis |
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