Transcription Profiling of NOD-like Receptors in the Human Cornea with Disease

• Published in: Ocular immunology and inflammation Vol. 25; no. 3; pp. 364 - 369
• Main Authors: Oh, Joo Youn, Ko, Jung Hwa, Ryu, Jin Suk, Lee, Hyun Ju, Kim, Mee Kum, Wee, Won Ryang
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.06.2017
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Adolescent; Adult; Aged; Aged, 80 and over; Apoptosis Regulatory Proteins - genetics; Cell Line; Cornea; Cornea - drug effects; Cornea - metabolism; Corneal Diseases - genetics; Female; Gene Expression Profiling; Gene Expression Regulation - physiology; human; Humans; lipopolysaccharide; Lipopolysaccharides - pharmacology; Male; Middle Aged; NLR Family, Pyrin Domain-Containing 3 Protein - genetics; NLRs; Nod1 Signaling Adaptor Protein - genetics; Nod2 Signaling Adaptor Protein - genetics; nucleotide-binding oligomerization domain (NOD)-like receptors; Real-Time Polymerase Chain Reaction; RNA, Messenger - genetics; Young Adult; Cornea; NLRs; lipopolysaccharide; nucleotide-binding oligomerization domain (NOD)-like receptors; human
• DOI: 10.3109/09273948.2015.1130844
• ISSN: 0927-3948

Purpose: To investigate the expression of nucleotide-binding oligomerization domain-like receptors (NLRs) in human corneas with disease and corneal cells. Methods: The expression of NOD1, NOD2, NLRP1, and NLRP3 was analyzed using real-time RT-PCR in (1) corneas with active infection, history of herpetic stromal keratitis (HSK), chronic allograft rejection, and limbal stem cell deficiency (LSCD), and (2) human corneal cells after lipopolysaccharide (LPS) stimulation. Healthy corneas and cells without LPS served as controls. Results: The mRNA levels of NOD2 and NLRP3 were increased in corneas with infection and HSK. Conversely, the levels of NOD1, NOD2, NLRP1, and NLRP3 transcripts were decreased in corneas with LSCD. In corneas with rejection, the expression of NOD1 and NLRP1 was downregulated. Corneal endothelial cells upregulated the expression of NOD2 and NLRP3 upon LPS. Conclusions: The changes in the NLR expression may reflect different susceptibility to infectious and non-infectious injuries in corneas with various diseases.