Transcription Profiling of NOD-like Receptors in the Human Cornea with Disease
Purpose: To investigate the expression of nucleotide-binding oligomerization domain-like receptors (NLRs) in human corneas with disease and corneal cells. Methods: The expression of NOD1, NOD2, NLRP1, and NLRP3 was analyzed using real-time RT-PCR in (1) corneas with active infection, history of herp...
Saved in:
Published in | Ocular immunology and inflammation Vol. 25; no. 3; pp. 364 - 369 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Taylor & Francis
01.06.2017
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Purpose: To investigate the expression of nucleotide-binding oligomerization domain-like receptors (NLRs) in human corneas with disease and corneal cells.
Methods: The expression of NOD1, NOD2, NLRP1, and NLRP3 was analyzed using real-time RT-PCR in (1) corneas with active infection, history of herpetic stromal keratitis (HSK), chronic allograft rejection, and limbal stem cell deficiency (LSCD), and (2) human corneal cells after lipopolysaccharide (LPS) stimulation. Healthy corneas and cells without LPS served as controls.
Results: The mRNA levels of NOD2 and NLRP3 were increased in corneas with infection and HSK. Conversely, the levels of NOD1, NOD2, NLRP1, and NLRP3 transcripts were decreased in corneas with LSCD. In corneas with rejection, the expression of NOD1 and NLRP1 was downregulated. Corneal endothelial cells upregulated the expression of NOD2 and NLRP3 upon LPS.
Conclusions: The changes in the NLR expression may reflect different susceptibility to infectious and non-infectious injuries in corneas with various diseases. |
---|---|
AbstractList | Purpose: To investigate the expression of nucleotide-binding oligomerization domain-like receptors (NLRs) in human corneas with disease and corneal cells.
Methods: The expression of NOD1, NOD2, NLRP1, and NLRP3 was analyzed using real-time RT-PCR in (1) corneas with active infection, history of herpetic stromal keratitis (HSK), chronic allograft rejection, and limbal stem cell deficiency (LSCD), and (2) human corneal cells after lipopolysaccharide (LPS) stimulation. Healthy corneas and cells without LPS served as controls.
Results: The mRNA levels of NOD2 and NLRP3 were increased in corneas with infection and HSK. Conversely, the levels of NOD1, NOD2, NLRP1, and NLRP3 transcripts were decreased in corneas with LSCD. In corneas with rejection, the expression of NOD1 and NLRP1 was downregulated. Corneal endothelial cells upregulated the expression of NOD2 and NLRP3 upon LPS.
Conclusions: The changes in the NLR expression may reflect different susceptibility to infectious and non-infectious injuries in corneas with various diseases. To investigate the expression of nucleotide-binding oligomerization domain-like receptors (NLRs) in human corneas with disease and corneal cells. The expression of NOD1, NOD2, NLRP1, and NLRP3 was analyzed using real-time RT-PCR in (1) corneas with active infection, history of herpetic stromal keratitis (HSK), chronic allograft rejection, and limbal stem cell deficiency (LSCD), and (2) human corneal cells after lipopolysaccharide (LPS) stimulation. Healthy corneas and cells without LPS served as controls. The mRNA levels of NOD2 and NLRP3 were increased in corneas with infection and HSK. Conversely, the levels of NOD1, NOD2, NLRP1, and NLRP3 transcripts were decreased in corneas with LSCD. In corneas with rejection, the expression of NOD1 and NLRP1 was downregulated. Corneal endothelial cells upregulated the expression of NOD2 and NLRP3 upon LPS. The changes in the NLR expression may reflect different susceptibility to infectious and non-infectious injuries in corneas with various diseases. PURPOSETo investigate the expression of nucleotide-binding oligomerization domain-like receptors (NLRs) in human corneas with disease and corneal cells.METHODSThe expression of NOD1, NOD2, NLRP1, and NLRP3 was analyzed using real-time RT-PCR in (1) corneas with active infection, history of herpetic stromal keratitis (HSK), chronic allograft rejection, and limbal stem cell deficiency (LSCD), and (2) human corneal cells after lipopolysaccharide (LPS) stimulation. Healthy corneas and cells without LPS served as controls.RESULTSThe mRNA levels of NOD2 and NLRP3 were increased in corneas with infection and HSK. Conversely, the levels of NOD1, NOD2, NLRP1, and NLRP3 transcripts were decreased in corneas with LSCD. In corneas with rejection, the expression of NOD1 and NLRP1 was downregulated. Corneal endothelial cells upregulated the expression of NOD2 and NLRP3 upon LPS.CONCLUSIONSThe changes in the NLR expression may reflect different susceptibility to infectious and non-infectious injuries in corneas with various diseases. |
Author | Lee, Hyun Ju Wee, Won Ryang Kim, Mee Kum Oh, Joo Youn Ko, Jung Hwa Ryu, Jin Suk |
Author_xml | – sequence: 1 givenname: Joo Youn surname: Oh fullname: Oh, Joo Youn email: jooyounoh77@gmail.com organization: Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, National University Hospital – sequence: 2 givenname: Jung Hwa surname: Ko fullname: Ko, Jung Hwa organization: Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, National University Hospital – sequence: 3 givenname: Jin Suk surname: Ryu fullname: Ryu, Jin Suk organization: Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, National University Hospital – sequence: 4 givenname: Hyun Ju surname: Lee fullname: Lee, Hyun Ju organization: Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, National University Hospital – sequence: 5 givenname: Mee Kum surname: Kim fullname: Kim, Mee Kum organization: Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, National University Hospital – sequence: 6 givenname: Won Ryang surname: Wee fullname: Wee, Won Ryang organization: Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, National University Hospital |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26902715$$D View this record in MEDLINE/PubMed |
BookMark | eNp9kMtO6zAURS0EgvL4BJCHd5JyHDtOPLuo5SUhQAjGluMeg7mJ3WunQvw9jVoYMtqTtfeW1iHZDTEgIacMppyBOgdV1lyJZloCq6aMcWiE2CETVgtRVFA3u2QyMsUIHZDDnN8BQCjF9slBKRWUNasm5P45mZBt8svBx0AfU3S-8-GVRkfvH-ZF5_8hfUKLyyGmTH2gwxvSm1VvAp3FFNDQDz-80bnPaDIekz1nuown2zwiL1eXz7Ob4u7h-nZ2cVdYLuVQGNvIloPjVtQtt07UCm0DLWLNQS2ahlsrFGBbcumglVZU1nCJDCuJFSv5Efmz2V2m-H-FedC9zxa7zgSMq6xZU0pZSWBijVYb1KaYc0Knl8n3Jn1qBnpUqb9V6lGl3qpc9862F6u2x8VP69vdGvi7AXxwMfXmI6ZuoQfz2cXk1latz-P-bx9fVYaEEw |
CitedBy_id | crossref_primary_10_1016_j_preteyeres_2021_101028 crossref_primary_10_3390_ijms24054309 crossref_primary_10_1016_j_lfs_2022_120881 crossref_primary_10_1016_j_exer_2021_108647 crossref_primary_10_1016_j_ajo_2019_01_002 crossref_primary_10_1097_ICO_0000000000003399 crossref_primary_10_1167_iovs_62_9_2 crossref_primary_10_1016_j_ajo_2018_01_017 crossref_primary_10_3390_ijms21030899 crossref_primary_10_1038_s41420_023_01511_0 crossref_primary_10_1038_s41598_024_51702_7 crossref_primary_10_1167_iovs_19_27144 crossref_primary_10_1016_j_ajo_2020_02_002 |
Cites_doi | 10.1038/cr.2012.56 10.1038/ni.1960 10.4049/jimmunol.1001854 10.1016/j.micinf.2010.05.006 10.1016/j.exer.2010.03.012 10.3109/08830185.2012.749400 10.3389/fimmu.2013.00393 10.1146/annurev.pathol.4.110807.092239 10.1371/journal.ppat.1000379 10.1016/j.immuni.2011.04.013 10.1073/pnas.0802726105 10.4049/jimmunol.1003761 10.1038/nm.2391 10.1186/1471-2148-9-202 10.1146/annurev-immunol-032712-095944 10.1016/j.immuni.2010.03.003 10.1002/ibd.22848 10.3346/jkms.2008.23.5.864 10.1038/nature04515 10.1152/physrev.00009.2014 10.1038/ni.1985 10.1016/j.preteyeres.2012.05.003 10.3109/09273948.2014.891754 10.1016/j.coi.2012.04.010 10.1002/mrd.20937 10.1097/01.ico.0000263127.84015.3f 10.1002/ibd.21478 10.1167/iovs.12-9724 10.1016/j.immuni.2014.12.010 10.1158/0008-5472.CAN-08-2061 |
ContentType | Journal Article |
Copyright | Taylor & Francis Group, LLC |
Copyright_xml | – notice: Taylor & Francis Group, LLC |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
DOI | 10.3109/09273948.2015.1130844 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine |
EISSN | 1744-5078 |
EndPage | 369 |
ExternalDocumentID | 10_3109_09273948_2015_1130844 26902715 1130844 |
Genre | Articles Journal Article |
GrantInformation_xml | – fundername: SNUH Research Fund grantid: 04-2012-0360 – fundername: Korean Health Technology R&D Project, Ministry of Health & Welfare grantid: HI14C2257 |
GroupedDBID | --- 00X 03L 0BK 0R~ 123 29N 36B 4.4 AAJNR AALUX AAMIU AAPUL AAPXX AAQRR AAWTL ABBKH ABDBF ABEIZ ABLKL ABPTK ABUPF ACENM ACFUF ACGEJ ACGFS ACLSK ADCVX ADFCX ADRBQ ADXPE AECIN AENEX AEOZL AEYQI AFKVX AFWLO AGDLA AGFJD AGRBW AGYJP AIJEM AIRBT AJWEG AKBVH ALIIL ALMA_UNASSIGNED_HOLDINGS ALQZU AMDAE BABNJ BLEHA BOHLJ CCCUG CS3 DKSSO DU5 EAP EBC EBD EBS EJD EMB EMK EMOBN EPL ESX F5P H13 HZ~ KRBQP KSSTO KWAYT KYCEM M4Z O9- P2P RNANH RVRKI SV3 TFDNU TFL TFW TUS UEQFS V1S ZA5 ~1N 53G 5VS AALIY AAORF ABJNI ABLIJ ABWCV ABXYU ABZEW ACIEZ ACKZS ADFOM ADFZZ AEIIZ AFLEI AJVHN ALYBC AWYRJ BRMBE CAG CGR COF CUY CVF CYYVM CZDIS DRXRE DWTOO ECM EIF JENTW LJTGL M44 NPM NUSFT QQXMO TBQAZ TDBHL TERGH TUROJ AAYXX CITATION 7X8 |
ID | FETCH-LOGICAL-c366t-ac86b30f3c47b3cf479ec80bee7309d883cc490eb236f0b6c45ca36e1e56e5123 |
ISSN | 0927-3948 |
IngestDate | Tue Aug 27 04:55:36 EDT 2024 Fri Aug 23 01:01:58 EDT 2024 Wed Oct 16 00:56:13 EDT 2024 Tue Jun 13 19:29:07 EDT 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Keywords | Cornea NLRs lipopolysaccharide nucleotide-binding oligomerization domain (NOD)-like receptors human |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c366t-ac86b30f3c47b3cf479ec80bee7309d883cc490eb236f0b6c45ca36e1e56e5123 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 26902715 |
PQID | 1826656014 |
PQPubID | 23479 |
PageCount | 6 |
ParticipantIDs | proquest_miscellaneous_1826656014 crossref_primary_10_3109_09273948_2015_1130844 informaworld_taylorfrancis_310_3109_09273948_2015_1130844 pubmed_primary_26902715 |
PublicationCentury | 2000 |
PublicationDate | 2017-06-01 |
PublicationDateYYYYMMDD | 2017-06-01 |
PublicationDate_xml | – month: 06 year: 2017 text: 2017-06-01 day: 01 |
PublicationDecade | 2010 |
PublicationPlace | England |
PublicationPlace_xml | – name: England |
PublicationTitle | Ocular immunology and inflammation |
PublicationTitleAlternate | Ocul Immunol Inflamm |
PublicationYear | 2017 |
Publisher | Taylor & Francis |
Publisher_xml | – name: Taylor & Francis |
References | CIT0030 Trinh L (CIT0036) 2008; 14 CIT0010 CIT0032 CIT0031 da Silva Correia J (CIT0008) 1840; 2006 CIT0012 CIT0034 CIT0011 CIT0033 Shin YJ (CIT0015) 2011; 17 Watanabe T (CIT0020) 2008; 118 CIT0014 CIT0013 CIT0016 CIT0018 CIT0019 CIT0021 Solomon A (CIT0017) 2000; 41 CIT0001 CIT0023 CIT0022 Benko S (CIT0035) 2008; 14 CIT0003 CIT0025 CIT0002 CIT0024 CIT0005 CIT0027 CIT0004 CIT0026 CIT0007 CIT0029 CIT0006 CIT0028 CIT0009 |
References_xml | – ident: CIT0007 doi: 10.1038/cr.2012.56 – ident: CIT0009 doi: 10.1038/ni.1960 – volume: 41 start-page: 2544 year: 2000 ident: CIT0017 publication-title: Invest Ophthalmol Vis Sci contributor: fullname: Solomon A – ident: CIT0023 doi: 10.4049/jimmunol.1001854 – ident: CIT0021 doi: 10.1016/j.micinf.2010.05.006 – volume: 118 start-page: 545 year: 2008 ident: CIT0020 publication-title: J Clin Invest contributor: fullname: Watanabe T – ident: CIT0012 doi: 10.1016/j.exer.2010.03.012 – ident: CIT0011 doi: 10.3109/08830185.2012.749400 – volume: 14 start-page: 1575 year: 2008 ident: CIT0035 publication-title: Mol Vis contributor: fullname: Benko S – ident: CIT0028 doi: 10.3389/fimmu.2013.00393 – ident: CIT0002 doi: 10.1146/annurev.pathol.4.110807.092239 – volume: 14 start-page: 1149 year: 2008 ident: CIT0036 publication-title: Mol Vis contributor: fullname: Trinh L – ident: CIT0027 doi: 10.1371/journal.ppat.1000379 – ident: CIT0022 doi: 10.1016/j.immuni.2011.04.013 – ident: CIT0024 doi: 10.1073/pnas.0802726105 – ident: CIT0019 doi: 10.4049/jimmunol.1003761 – ident: CIT0026 doi: 10.1038/nm.2391 – ident: CIT0006 doi: 10.1186/1471-2148-9-202 – ident: CIT0032 doi: 10.1146/annurev-immunol-032712-095944 – ident: CIT0030 doi: 10.1016/j.immuni.2010.03.003 – volume: 17 start-page: 3371 year: 2011 ident: CIT0015 publication-title: Mol Vis contributor: fullname: Shin YJ – ident: CIT0025 doi: 10.1002/ibd.22848 – ident: CIT0013 doi: 10.3346/jkms.2008.23.5.864 – ident: CIT0033 doi: 10.1038/nature04515 – ident: CIT0001 doi: 10.1152/physrev.00009.2014 – ident: CIT0004 doi: 10.1038/ni.1985 – ident: CIT0010 doi: 10.1016/j.preteyeres.2012.05.003 – ident: CIT0014 doi: 10.3109/09273948.2014.891754 – ident: CIT0029 doi: 10.1016/j.coi.2012.04.010 – ident: CIT0005 doi: 10.1002/mrd.20937 – volume: 2006 issue: 103 year: 1840 ident: CIT0008 publication-title: Proc Natl Acad Sci USA contributor: fullname: da Silva Correia J – ident: CIT0034 doi: 10.1097/01.ico.0000263127.84015.3f – ident: CIT0031 doi: 10.1002/ibd.21478 – ident: CIT0016 doi: 10.1167/iovs.12-9724 – ident: CIT0003 doi: 10.1016/j.immuni.2014.12.010 – ident: CIT0018 doi: 10.1158/0008-5472.CAN-08-2061 |
SSID | ssj0004991 |
Score | 2.2428164 |
Snippet | Purpose: To investigate the expression of nucleotide-binding oligomerization domain-like receptors (NLRs) in human corneas with disease and corneal cells.... To investigate the expression of nucleotide-binding oligomerization domain-like receptors (NLRs) in human corneas with disease and corneal cells. The... PURPOSETo investigate the expression of nucleotide-binding oligomerization domain-like receptors (NLRs) in human corneas with disease and corneal... |
SourceID | proquest crossref pubmed informaworld |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 364 |
SubjectTerms | Adaptor Proteins, Signal Transducing - genetics Adolescent Adult Aged Aged, 80 and over Apoptosis Regulatory Proteins - genetics Cell Line Cornea Cornea - drug effects Cornea - metabolism Corneal Diseases - genetics Female Gene Expression Profiling Gene Expression Regulation - physiology human Humans lipopolysaccharide Lipopolysaccharides - pharmacology Male Middle Aged NLR Family, Pyrin Domain-Containing 3 Protein - genetics NLRs Nod1 Signaling Adaptor Protein - genetics Nod2 Signaling Adaptor Protein - genetics nucleotide-binding oligomerization domain (NOD)-like receptors Real-Time Polymerase Chain Reaction RNA, Messenger - genetics Young Adult |
Title | Transcription Profiling of NOD-like Receptors in the Human Cornea with Disease |
URI | https://www.tandfonline.com/doi/abs/10.3109/09273948.2015.1130844 https://www.ncbi.nlm.nih.gov/pubmed/26902715 https://search.proquest.com/docview/1826656014 |
Volume | 25 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3da9swEBdZCmMvY9_r1g0N9lacOdaHrce2WQmDJmNroW_GkmVWttqlsxntv9B_uneW7DilK-teTDDRWfb9dDrdJyEfZZJk0k51EMVTFfAcdDilwzwQmREWPb2qLZl_sJDzI_7lWByPRleDqKWm1hNzeWteyf9wFe4BXzFL9h6c7YnCDfgN_IUrcBiu_8Zj3Gj6Zf-1bb_to5gXy1nw6-QndmXAwBVsqeMjGp3Zfq86L23mzLCzgZPG66lLH56K2SPDKk0FAOh0zXm__OGM8NU2Co5efjuHDkiS7fmfXvJ_u2ja2zCT702fIuRjgeYXTQkjhmaIabwKl-rsiRFIK-XKZk6sk6Yx5wEonMlQ3Lo8Zw8rNpCdzJUz99swcx1cbkp45gqk4tPwYRibJ7AvTZhwvtrSOjf-jZ2ujz-Ekw8SSjsyKZJJPZkHZCOKlRBjsrGzO9vdX6XZKteAsXtTlxCGhD7dOp81VWetEO7fjzOtWnP4hDz25xG648D1lIxs-Yw8PPARF8_JYg1jtMcYrQraYYz2GKMnJQWM0RZj1GGMIsaox9gLcrT_-XBvHvgeHIFhUtZBZhKpWVgww2PNTMFjZU0Samtha1B5kjBjuAqtjpgsQi0NFyZjsPqtkBaUSfaSjMuqtK8JjTJeGCNEkSnJLdeZap3gRZ4njIdRvkkm3edKz1yplfRORm0SNfyoad3auArXkAbH3Tn2Q8eBFAQqesmy0lbN7xQP3FiRagr_eeVY008nkioEoSbe3Heqb8mj1aLZIuP6vLHvQJut9XuPsmvw0JQH |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | EBSCOhost |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Transcription+Profiling+of+NOD-like+Receptors+in+the+Human+Cornea+with+Disease&rft.jtitle=Ocular+immunology+and+inflammation&rft.au=Oh%2C+Joo+Youn&rft.au=Ko%2C+Jung+Hwa&rft.au=Ryu%2C+Jin+Suk&rft.au=Lee%2C+Hyun+Ju&rft.date=2017-06-01&rft.issn=0927-3948&rft.eissn=1744-5078&rft.volume=25&rft.issue=3&rft.spage=364&rft.epage=369&rft_id=info:doi/10.3109%2F09273948.2015.1130844&rft.externalDBID=n%2Fa&rft.externalDocID=10_3109_09273948_2015_1130844 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0927-3948&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0927-3948&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0927-3948&client=summon |