Intramuscular DNA vaccine provides protection in non-human primate and mouse models of SARS-CoV-2

• Published in: Frontiers in immunology Vol. 16; p. 1589584
• Main Authors: Sood, Subeena, Gary, Ebony N., Matar, Majed, Kim, Jessica, Hojecki, Casey E., Warner, Bryce, Vendramelli, Robert, Truong, Thang, Smith, Alanna, Rice, Jennifer, Sparks, Jeff, DeSalvo, Michael, Henderson, John, Rogers, Joseph A., Sharma, Ankur, Pessaint, Laurent, Iavarone, Carlo A., Kobasa, Darwyn, Boyer, Jean D., Lindborg, Stacy, Anwer, Khursheed
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 2025
• Subjects: Animals; Antibodies, Neutralizing - immunology; Antibodies, Viral - blood; Antibodies, Viral - immunology; COVID-19 - immunology; COVID-19 - prevention & control; COVID-19 Vaccines - administration & dosage; COVID-19 Vaccines - immunology; Disease Models, Animal; DNA; Female; Humans; Immunity, Humoral; immunology; Injections, Intramuscular; Mice; Mice, Inbred BALB C; plasmid; primate; SARS-CoV-2 - immunology; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - immunology; vaccine; Vaccines, DNA - administration & dosage; Vaccines, DNA - immunology; Viral Load; primate; SARS-CoV-2; vaccine; DNA; plasmid; immunology; mice

Nucleic acid vaccine approaches have proven successful in the context of the SARS-CoV-2 pandemic, however challenges with delivery remain. Here we describe PlaCCine, a DNA-based vaccine platform that utilizes a device- and vector-free chemical delivery system. This system includes a DNA plasmid enco...