Psoralen Isolated from the Roots of Dorstenia psilurus Welw. Modulate Th1/Th2 Cytokines and Inflammatory Enzymes in LPS-Stimulated RAW 264.7 Macrophages

• Published in: Mediators of inflammation Vol. 2024; no. 1; p. 8233689
• Main Authors: Isa, Adamu Imam, Fouotsa, Hugues, Mohammed, Osama A, Alghamdi, Mushabab, Adamu, Bappa, Alfaifi, Jaber, Jibo, Abubakar Mohammed, Alamri, Mohannad Mohammed Saleh, Khan, Sameer, Adam, Masoud Ishag Elkhalifa, Alqarni, Abdullah Ali, Mohamed, Mohamed O'haj, Ateba, Joël Eddy Terence, Dzoyem, Jean Paul
• Format: Journal Article
• Language: English
• Published: United States Hindawi Limited 2024; Wiley
• Subjects: Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Cell proliferation; Chromatography; Cytokines; Cytokines - metabolism; Dorstenia; Enzymes; Ficusin - chemistry; Ficusin - pharmacology; Herbal medicine; Infections; Inflammation; Inflammatory diseases; Interleukins; Lipopolysaccharides - pharmacology; Lipoxygenase; Lymphocytes T; Macrophages; Macrophages - drug effects; Macrophages - metabolism; Medical research; Medicinal plants; Mice; Natural products; Nitric oxide; Nitric Oxide - metabolism; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Roots - chemistry; Prostaglandin endoperoxide synthase; Psoralen; RAW 264.7 Cells; Th1 Cells - drug effects; Th1 Cells - metabolism; Th2 Cells - drug effects; Th2 Cells - metabolism; Tumor necrosis factor-α; Viral infections; γ-Interferon; Brazil; Mexico; Cameroon; Panama; Ethiopia; Germany
• ISSN: 0962-9351; 1466-1861; 1466-1861
• DOI: 10.1155/2024/8233689

is a widely used plant spice in traditional African medicine to treat pain-related conditions. However, the anti-inflammatory mechanisms underlying this activity and the main active ingredients of have not yet been fully characterized. This study aimed to isolate and identify the main active anti-inflammatory constituents of the extract and to investigate the underlying anti-inflammatory mechanisms in murine macrophages. Chromatographic techniques and spectroscopic data were used for compound isolation and structure elucidation. The Griess reagent method and the ferrous oxidation-xylenol orange assay were used to evaluate the inhibition of NO production and 15-lipoxygenase activity, respectively. Cyclooxygenase activity was assessed using the fluorometric COX activity assay kit, and Th1/Th2 cytokine measurement was performed using a flow cytometer. The results indicated that the extract and fractions of inhibit NO production and proliferation of RAW 264.7 macrophage cells. Bioguided fractionation led to the identification of psoralen, a furocoumarin, as the main bioactive anti-inflammatory compound. Psoralen inhibited NO production and 15-lipoxygenase activity and reduced pro-inflammatory Th1 cytokines (IFN- , TNF- , and IL-2) while increasing the secretion of anti-inflammatory cytokines (IL-4, IL-6, and IL-10) in activated RAW 264.7 macrophage cells. The encouraging results obtained in this study suggest that psoralen-based multiple modulation strategies could be a useful approach to address the treatment of inflammatory diseases.