Study of complexation of single-stranded poly(rA) and poly(rU) with methylene blue

• Published in: Journal of biomolecular structure & dynamics Vol. 41; no. 24; pp. 15320 - 15327
• Main Authors: Vardevanyan, Poghos O., Antonyan, Ara P., Movsisyan, Zvart H., Parsadanyan, Marine A., Shahinyan, Mariam A., Grigoryan, Karine R., Shilajyan, Hasmik A.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 29.12.2023
• Subjects: absorption spectra; binding mode; fluorescence spectra; Methylene blue; synthetic polyribonucleotides; synthetic polyribonucleotides; binding mode; fluorescence spectra; absorption spectra; Methylene blue
• ISSN: 0739-1102; 1538-0254
• DOI: 10.1080/07391102.2023.2189475

To reveal the effect of DNA- or RNA-specific low-molecular compounds on cellular processes on the molecular level, we have carried out the studies with the application of spectroscopic methods. It is necessary for the understanding of structural-functional properties of nucleic acids in cell. In this work the interaction of DNA-specific thiazine dye methylene blue (MB) with synthetic polynucleotides poly(rA) and poly(rU) was studied. The interaction of MB with synthetic polyribonucleotides poly(rA) and poly(rU) was examined in the solution with high ionic strength in a wide phosphate-to-dye (P/D) range, using the absorption and fluorescence spectroscopies, as well as the fluorescence 2D spectra and 3D spectra analyses were given. Values of the fluorescence quenching constants for the complexes of MB with poly(rA) and poly(rU) were calculated (K SV is the Stern-Volmer quenching constant). Two different modes of MB binding to single-stranded (ss-) poly(rA) and poly(rU) and to their hybrid double-stranded (ds-) structure - poly(rA)-poly(rU) were identified. This ligand binds to ss-poly(rA) and poly(rA)-poly(rU) by semi-intercalation and electrostatic modes, but to ss-poly(rU) the prevailing mode is the electrostatic interaction. Communicated by Ramaswamy H. Sarma