Efficient activity screening of new glucuronoyl esterases using a pNP-based assay

• Published in: Enzyme and microbial technology Vol. 178; p. 110444
• Main Authors: Madsen, Michael S., Martins, Pedro A., Agger, Jane W.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2024
• Subjects: Activity screening; CE15; Colorimetry - methods; Enzyme Assays - methods; Esterases - chemistry; Esterases - genetics; Esterases - metabolism; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Glucuronoyl esterases; Hydrolysis; Lignin - metabolism; Lignin carbohydrate complexes; Nitrophenols - metabolism; Recombinant expression; Specific activity; Substrate Specificity; Activity screening; Specific activity; Recombinant expression; CE15; Lignin carbohydrate complexes; Glucuronoyl esterases
• ISSN: 0141-0229; 1879-0909; 1879-0909
• DOI: 10.1016/j.enzmictec.2024.110444

Glucuronoyl esterases (CE15, EC 3.1.1.117) catalyze the hydrolysis of ester bonds between lignin and carbohydrates in lignocellulose. They are widespread within fungi and bacteria, and are subjects to research interest due to their potential applicability in lignocellulose processing. Identifying ne...