Tracking DOT1L methyltransferase activity by stable isotope labelling using a selective synthetic co-factor

• Published in: Communications chemistry Vol. 7; no. 1; pp. 145 - 7
• Main Authors: Trainor, Nicole, Whitwell, Harry J., Jiménez, Beatriz, Addison, Katie, Leonidou, Emily, DiMaggio, Peter A., Fuchter, Matthew J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 27.06.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/92/475; 631/92/96; 639/638/11/296; 639/638/92; 82/58; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Deoxyribonucleic acid; DNA; Epigenetics; Gene expression; Gene sequencing; Histones; Labels; Lysine; Mass spectrometry; Methionine; Methylation; Proteins; Proteomics; Redundancy; Substrates; Tracking

Epigenetic processes influence health and disease through mechanisms which alter gene expression. In contrast to genetic changes which affect DNA sequences, epigenetic marks include DNA base modifications or post-translational modification (PTM) of proteins. Histone methylation is a prominent and ve...