DNA polymerase delta is required for the replication feedback control of cell cycle progression in Schizosaccharomyces pombe

• Published in: Molecular & general genetics Vol. 246; no. 5; p. 561
• Main Authors: Francesconi, S, Recondo, A.M. de, Baldacci, G. (Centre National da la Recherche Scientifique, Villejuif (France). Inst. de Recherches sur le Cancer. Laboratoire de Biologie Moleculaire de la Replication)
• Format: Journal Article
• Language: English
• Published: Germany 01.09.1995
• Subjects: ADN; Cdc20 Proteins; Cell cycle checkpoints; Cell Cycle Proteins; Checkpoint Kinase 1; CONTROLE DE CROISSANCE; DIVISION CELLULAIRE; DIVISION CELULAR; DNA polymerase delta; DNA Polymerase III; DNA replication; DNA Replication - physiology; DNA-Directed DNA Polymerase - physiology; Feedback; Fission yeast; Fungal Proteins - genetics; Genes, Fungal - genetics; Genes, Lethal; Mitosis; Pflanzenzuechtung; POLIMERIZACION; POLYMERISATION; Protein Kinases - genetics; Radiation Tolerance; REGULACION DEL CRECIMIENTO; S Phase - genetics; Saccharomyces cerevisiae Proteins; Schizosaccharomyces - enzymology; Schizosaccharomyces - genetics; Schizosaccharomyces - growth & development; SCHIZOSACCHAROMYCES POMBE; Schizosaccharomyces pombe Proteins; Sequence Deletion - physiology; Temperature
• ISSN: 0026-8925; 1432-1874
• DOI: 10.1007/BF00298962

DNA replication and DNA repair are essential cell cycle steps ensuring correct transmission of the genome. The feedback replication control system links mitosis to completion of DNA replication and partially overlaps the radiation checkpoint control. Deletion of the chk1/rad27 gene abolishes the radiation but not the replication feedback control. Thermosensitive mutations in the DNA polymerase delta, cdc18 or cdc20 genes lead cells to arrest in the S phase of the cell cycle. Strains carrying any of these mutations enter lethal mitosis in the absence of the radiation checkpoint chk1/rad27. these data are interpreted as an indication that an assembled replisome is essential for replication dependent control of mitosis and it is proposed that the arrest of the cell cycle in the thermosensitive mutants is due to the chk1/rad71 pathway, which monitors directly DNA for signs of damage.