Diversity of Helicobacter pylori VacA and CagA genes and relationship to VacA and CagA protein expression, cytotoxin production, and associated diseases
The vacuolating cytotoxin and the cytotoxin-associated protein, encoded by vacA and cagA, respectively, are important virulence determinants of Helicobacter pylori. Sixty-five H. pylori strains were isolated from dyspeptic patients (19 with peptic ulcer disease, 43 with chronic gastritis, and 3 with...
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Published in | Journal of clinical microbiology Vol. 36; no. 4; pp. 944 - 948 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Society for Microbiology
01.04.1998
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Subjects | |
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Abstract | The vacuolating cytotoxin and the cytotoxin-associated protein, encoded by vacA and cagA, respectively, are important virulence determinants of Helicobacter pylori. Sixty-five H. pylori strains were isolated from dyspeptic patients (19 with peptic ulcer disease, 43 with chronic gastritis, and 3 with gastric cancer) and studied for differences in the vacA and cagA genes and their relationship to VacA and CagA expression, cytotoxin activity, and the clinical outcome of infection. By PCR, fifty-four (83.1%) of 65 strains had the vacA signal sequence genotype s1 and only 10 (15.4%) had the type s2. After primer modification, the vacA middle-region types m1 and m2 were detected in 24 (36.9%) and 41 (63.1%) strains, respectively. The combinations s1-m2 (31 [47.7%]) and s1-m1 (23 [35.4%]) occurred more frequently than s2-m2 (10 [15.4%]) (P = 0.01). No strain with the combination s2-m1 was found. All 19 patients with peptic ulcers harbored type s1 strains, in contrast to 32 (74.4%) of 43 patients with gastritis (P = 0.02). The vacA genotype s1 was associated with the presence of cagA (P < 0.0001), VacA expression (P < 0.0001), and cytotoxin activity (P = 0.003). The cagA gene was detectable in 48 (73.8%) of 65 isolates and present in 16 (84.2%) of 19 ulcer patients and 29 (67.4%) of 43 patients with gastritis (P = 0.17). The vacA genotypes of German H. pylori isolates are identical to those previously reported. H. pylori strains of vacA type s1 are associated with the occurrence of peptic ulceration and the presence of cagA, cytotoxin activity, and VacA expression. |
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AbstractList | The vacuolating cytotoxin and the cytotoxin-associated protein, encoded by vacA and cagA, respectively, are important virulence determinants of Helicobacter pylori. Sixty-five H. pylori strains were isolated from dyspeptic patients (19 with peptic ulcer disease, 43 with chronic gastritis, and 3 with gastric cancer) and studied for differences in the vacA and cagA genes and their relationship to VacA and CagA expression, cytotoxin activity, and the clinical outcome of infection. By PCR, fifty-four (83.1%) of 65 strains had the vacA signal sequence genotype s1 and only 10 (15.4%) had the type s2. After primer modification, the vacA middle-region types m1 and m2 were detected in 24 (36.9%) and 41 (63.1%) strains, respectively. The combinations s1-m2 (31 [47.7%]) and s1-m1 (23 [35.4%]) occurred more frequently than s2-m2 (10 [15.4%]) (P = 0.01). No strain with the combination s2-m1 was found. All 19 patients with peptic ulcers harbored type s1 strains, in contrast to 32 (74.4%) of 43 patients with gastritis (P = 0.02). The vacA genotype s1 was associated with the presence of cagA (P < 0.0001), VacA expression (P < 0.0001), and cytotoxin activity (P = 0.003). The cagA gene was detectable in 48 (73.8%) of 65 isolates and present in 16 (84.2%) of 19 ulcer patients and 29 (67.4%) of 43 patients with gastritis (P = 0.17). The vacA genotypes of German H. pylori isolates are identical to those previously reported. H. pylori strains of vacA type s1 are associated with the occurrence of peptic ulceration and the presence of cagA, cytotoxin activity, and VacA expression. The vacuolating cytotoxin and the cytotoxin-associated protein, encoded by vacA and cagA , respectively, are important virulence determinants of Helicobacter pylori . Sixty-five H. pylori strains were isolated from dyspeptic patients (19 with peptic ulcer disease, 43 with chronic gastritis, and 3 with gastric cancer) and studied for differences in the vacA and cagA genes and their relationship to VacA and CagA expression, cytotoxin activity, and the clinical outcome of infection. By PCR, fifty-four (83.1%) of 65 strains had the vacA signal sequence genotype s1 and only 10 (15.4%) had the type s2. After primer modification, the vacA middle-region types m1 and m2 were detected in 24 (36.9%) and 41 (63.1%) strains, respectively. The combinations s1-m2 (31 [47.7%]) and s1-m1 (23 [35.4%]) occurred more frequently than s2-m2 (10 [15.4%]) ( P = 0.01). No strain with the combination s2-m1 was found. All 19 patients with peptic ulcers harbored type s1 strains, in contrast to 32 (74.4%) of 43 patients with gastritis ( P = 0.02). The vacA genotype s1 was associated with the presence of cagA ( P < 0.0001), VacA expression ( P < 0.0001), and cytotoxin activity ( P = 0.003). The cagA gene was detectable in 48 (73.8%) of 65 isolates and present in 16 (84.2%) of 19 ulcer patients and 29 (67.4%) of 43 patients with gastritis ( P = 0.17). The vacA genotypes of German H. pylori isolates are identical to those previously reported. H. pylori strains of vacA type s1 are associated with the occurrence of peptic ulceration and the presence of cagA , cytotoxin activity, and VacA expression. ABSTRACT The vacuolating cytotoxin and the cytotoxin-associated protein, encoded by vacA and cagA , respectively, are important virulence determinants of Helicobacter pylori . Sixty-five H. pylori strains were isolated from dyspeptic patients (19 with peptic ulcer disease, 43 with chronic gastritis, and 3 with gastric cancer) and studied for differences in the vacA and cagA genes and their relationship to VacA and CagA expression, cytotoxin activity, and the clinical outcome of infection. By PCR, fifty-four (83.1%) of 65 strains had the vacA signal sequence genotype s1 and only 10 (15.4%) had the type s2. After primer modification, the vacA middle-region types m1 and m2 were detected in 24 (36.9%) and 41 (63.1%) strains, respectively. The combinations s1-m2 (31 [47.7%]) and s1-m1 (23 [35.4%]) occurred more frequently than s2-m2 (10 [15.4%]) ( P = 0.01). No strain with the combination s2-m1 was found. All 19 patients with peptic ulcers harbored type s1 strains, in contrast to 32 (74.4%) of 43 patients with gastritis ( P = 0.02). The vacA genotype s1 was associated with the presence of cagA ( P < 0.0001), VacA expression ( P < 0.0001), and cytotoxin activity ( P = 0.003). The cagA gene was detectable in 48 (73.8%) of 65 isolates and present in 16 (84.2%) of 19 ulcer patients and 29 (67.4%) of 43 patients with gastritis ( P = 0.17). The vacA genotypes of German H. pylori isolates are identical to those previously reported. H. pylori strains of vacA type s1 are associated with the occurrence of peptic ulceration and the presence of cagA , cytotoxin activity, and VacA expression. |
Author | SIEG, A RUDI, J MAIWALD, M GALLE, P. R STREMMEL, W KOLB, C KUCK, D |
AuthorAffiliation | Department of Medicine, Division of Gastroenterology, 1 and Institute of Microbiology, 2 University of Heidelberg, Heidelberg, Germany |
AuthorAffiliation_xml | – name: Department of Medicine, Division of Gastroenterology, 1 and Institute of Microbiology, 2 University of Heidelberg, Heidelberg, Germany |
Author_xml | – sequence: 1 givenname: J surname: RUDI fullname: RUDI, J organization: Department of Medicine, Division of Gastroenterology, University of Heidelberg, Heidelberg, Germany – sequence: 2 givenname: C surname: KOLB fullname: KOLB, C organization: Department of Medicine, Division of Gastroenterology, University of Heidelberg, Heidelberg, Germany – sequence: 3 givenname: M surname: MAIWALD fullname: MAIWALD, M organization: Institute of Microbiology, University of Heidelberg, Heidelberg, Germany – sequence: 4 givenname: D surname: KUCK fullname: KUCK, D organization: Department of Medicine, Division of Gastroenterology, University of Heidelberg, Heidelberg, Germany – sequence: 5 givenname: A surname: SIEG fullname: SIEG, A organization: Department of Medicine, Division of Gastroenterology, University of Heidelberg, Heidelberg, Germany – sequence: 6 givenname: P. R surname: GALLE fullname: GALLE, P. R organization: Department of Medicine, Division of Gastroenterology, University of Heidelberg, Heidelberg, Germany – sequence: 7 givenname: W surname: STREMMEL fullname: STREMMEL, W organization: Department of Medicine, Division of Gastroenterology, University of Heidelberg, Heidelberg, Germany |
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Keywords | Human Prevalence Genetic variability Spirillales Spirillaceae Genotype Gene expression Gastritis Pathogenicity Toxin Helicobacter pylori DNA Molecular epidemiology Digestive diseases Bacteria Anastomotic ulcer Gastric disease |
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Notes | Corresponding author. Mailing address: Department of Medicine, Division of Gastroenterology, University of Heidelberg, Bergheimerstr. 58, 69115 Heidelberg, Germany. Phone: 49 6221-568611. Fax: 49 6221-565255. E-mail: jochen_rudi@krzmail.krz.uni-heidelberg.de |
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Snippet | The vacuolating cytotoxin and the cytotoxin-associated protein, encoded by vacA and cagA, respectively, are important virulence determinants of Helicobacter... ABSTRACT The vacuolating cytotoxin and the cytotoxin-associated protein, encoded by vacA and cagA , respectively, are important virulence determinants of... The vacuolating cytotoxin and the cytotoxin-associated protein, encoded by vacA and cagA , respectively, are important virulence determinants of Helicobacter... |
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SubjectTerms | Antigens, Bacterial Bacterial diseases Bacterial diseases of the digestive system and abdomen Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Bacteriology Biological and medical sciences Cytotoxins - biosynthesis Cytotoxins - genetics Cytotoxins - toxicity Fundamental and applied biological sciences. Psychology Genes, Bacterial Genotype HeLa Cells Helicobacter pylori - genetics Helicobacter pylori - pathogenicity Human bacterial diseases Humans Infectious diseases Medical sciences Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains |
Title | Diversity of Helicobacter pylori VacA and CagA genes and relationship to VacA and CagA protein expression, cytotoxin production, and associated diseases |
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