Photobinding of Tiaprofenic Acid and Suprofen to Proteins and Cells: A Combined Study Using Radiolabeling, Antibodies and Laser Flash Photolysis of Model Bichromophores

• Published in: Photochemistry and photobiology Vol. 68; no. 5; pp. 660 - 665
• Main Authors: Castell, José V., Hernández, Daniel, Gómez-Lechón, María José, Lahoz, Agustín, Miranda, Miguel A., Morera, Isabel M., Perez-Prieto, Julia, Sarabia, Zaideth
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.11.1998
• Subjects: 3T3 Cells; Animals; Anti-Inflammatory Agents, Non-Steroidal - chemistry; Anti-Inflammatory Agents, Non-Steroidal - pharmacokinetics; Cell Membrane - metabolism; Kinetics; Mice; Nuclear Magnetic Resonance, Biomolecular; Photolysis; Photosensitizing Agents - chemistry; Photosensitizing Agents - pharmacokinetics; Propionates - chemistry; Propionates - pharmacokinetics; Protein Binding; Rabbits; Serum Albumin - chemistry; Serum Albumin - metabolism; Suprofen - chemistry; Suprofen - pharmacokinetics

Drug photoallergy is a matter of current concern. It involves the formation of drug‐protein photoadducts (pho‐toantigens) that may ultimately trigger an immunological response. Tyrosine residues appear to be key binding sites in proteins. The present work has investigated the photobinding of tiaprof...