Allergenicity potential of red kidney bean (Phaseolus vulgaris L.) proteins in orally treated BALB/c mice and passively sensitized RBL-2H3 cells

• Published in: Cellular immunology Vol. 284; no. 1-2; pp. 37 - 44
• Main Authors: Kumar, Sandeep, Sharma, Akanksha, Verma, Alok K., Chaudhari, B.P., Das, Mukul, Jain, S.K., Dwivedi, Premendra D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.07.2013
• Subjects: Allergic mediators; Allergy; Anaphylaxis - immunology; Animals; beta-N-Acetylhexosaminidases - analysis; beta-N-Acetylhexosaminidases - immunology; Blotting, Western; Cell Line, Tumor; Female; Food Hypersensitivity - immunology; GATA3 Transcription Factor - analysis; GATA3 Transcription Factor - immunology; Legumes; Mice; Mice, Inbred BALB C; NFATC Transcription Factors - analysis; NFATC Transcription Factors - immunology; Phaseolus - immunology; Plant Proteins - immunology; Proto-Oncogene Proteins c-maf - analysis; Proto-Oncogene Proteins c-maf - immunology; Random Allocation; Rats; Specific Pathogen-Free Organisms; T-Box Domain Proteins - analysis; T-Box Domain Proteins - immunology; Th1 Cells - immunology; Th2 Cells - immunology; Thymic stromal lymphopoietin; Transcription factors; Legumes; Allergy; Transcription factors; Allergic mediators; Thymic stromal lymphopoietin
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/j.cellimm.2013.07.001

•Red kidney bean proteins following oral exposure induce anaphylactic symptoms.•Enhanced levels of TSLP was accompanied with anaphylactic symptoms.•Vascular permeability increased with red kidney bean proteins exposure.•Positive PCA indicated an IgE mediated reactions induced by red kidney bean proteins.•Up regulated expressions of GATA-3, STAT-6, T-bet, c-MAF and NFAT in RBL-2H3 cells following red kidney bean proteins exposure. Red kidney bean (Phaseolus vulgaris L.) is one the most commonly consumed legumes that requires an in depth understanding of its allergenicity. Therefore, the aim of this study was to explore the allergenicity of red kidney bean proteins following oral exposure in BALB/c mice and elucidate the levels of Th1/Th2 transcription factors induced by red kidney bean proteins in rat basophilic leukemia cells (RBL-2H3 cells) passively sensitized with the sera of red kidney bean sensitized mice. Red kidney bean proteins showed enhanced levels of total and specific IgE, anaphylactic symptoms, thymic stromal lymphopoietin (TSLP) and peritoneal albumin over control. Enhanced release of β-hexosaminidase along with up regulated expressions of GATA-3, STAT-6, T-bet, c-MAF and NFAT were observed in the RBL-2H3 cells exposed with red kidney bean proteins when compared to that of the controls. Taken together, exposure of red kidney bean proteins may cause allergic symptoms in mice and the ambivalent effect on Th2/Th1 transcription factors in RBL-2H3 cells.