Simultaneous removal of calcium, fluoride, nickel, and nitrate using microbial induced calcium precipitation in a biological immobilization reactor

• Published in: Journal of hazardous materials Vol. 416; p. 125776
• Main Authors: Liu, Jiaran, Ali, Amjad, Su, Junfeng, Wu, Zizhen, Zhang, Ruijie, Xiong, Renbo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.08.2021
• Subjects: Biofilm reactor; Biomineralization; Defluoridation; Denitrification; Ni2+ removal; Biomineralization; Defluoridation; Denitrification; Biofilm reactor; Ni2+ removal; Ni(2+) removal
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2021.125776

In this research, an immobilized biofilm reactor was established for the simultaneous removal of calcium (Ca2+), fluoride (F-), nickel (Ni2+), and nitrate (NO3--N) by microbial induced calcium precipitation (MICP). The operating parameters of the reactor, hydraulic retention time (HRT: 4, 8, and 12 h), influent Ca2+ concentration (36.0, 108.0, and 180.0 mg L−1), and influent Ni2+ concentration (0.0, 3.0, and 6.0 mg L−1) were discussed. Under the HRT of 12 h, influent Ca2+ concentration of 180.0 mg L−1, and influent Ni2+ concentration of 3.0 mg L−1, the removal ratios of Ca2+, F-, Ni2+, and NO3--N reached 45.31%, 79.55%, 85.11%, and 55.29%, respectively, which was the reactor stable operation performance. The SEM revealed the morphology of calcium-precipitated bio-crystals. XPS showed the Ca2+ and Ni2+ precipitate components and XRD further revealed the formation of CaCO3, Ca5(PO4)3OH, and NiCO3 precipitation. Nitrogen (N2) was the main gas produced in the reactor. Fluorescence spectroscopy manifested that extracellular polymers played an important role in the organism nucleation. High-throughput sequencing exhibited that Acinetobacter sp. H12 was the dominant bacterial group. This study provided a new insight for simultaneous remediation of Ca2+, F-, Ni2+, and NO3--N in water bodies. [Display omitted] •Biological immobilization reactor simultaneous removed Ca2+, F-, Ni2+, and NO3--N by MICP.•The removal performance of Ca2+, F-, and Ni2+ in the reactor was good under the specific parameters.•XPS and XRD proved the removal of F- and Ca2+ in the form of Ca5(PO4)3F and CaF2.•Ni2+ was removed in the form of NiCO3 by homogeneous substitution.•Calcium precipitating strain H12 was the main bacterium in the reactor.