Genetic engineering of CHO cells producing human interferon-gamma by transfection of sialyltransferases

• Published in: Glycoconjugate journal Vol. 17; no. 12; pp. 895 - 904
• Main Authors: Fukuta, K, Yokomatsu, T, Abe, R, Asanagi, M, Makino, T
• Format: Journal Article
• Language: English
• Published: United States 01.12.2000
• Subjects: Animals; Carbohydrate Conformation; Carbohydrate Sequence; CHO Cells; Chromatography, High Pressure Liquid; Clone Cells - enzymology; Clone Cells - metabolism; Cloning, Molecular; Cricetinae; Genetic Engineering - methods; Humans; Interferon-gamma - biosynthesis; Interferon-gamma - chemistry; Mice; Molecular Sequence Data; N-Acetylneuraminic Acid - metabolism; Polymerase Chain Reaction; Rats; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Sialyltransferases - genetics; Sialyltransferases - metabolism; Transfection

Natural human interferon-gamma (hIFN-gamma) contains mainly biantennary complex-type sugar chains. We previously remodeled the branch structures of N-glycans on hIFN-gamma in Chinese hamster ovary (CHO) cells by overexpressing UDP-N-acetylglucosamine: alpha1,6-D-mannoside beta1,6-N-acetylglucosaminy...