Certification and stability assessment of recombinant human growth hormone as a certified reference material for protein quantification

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1126-1127; p. 121732
• Main Authors: Tran, Thi Thanh Huong, Kim, Juok, Rosli, Nordiana, Mok, Inkyu, Oh, Kyong Hwa, Lee, Hwashim, Hong, Seon-Pyo, Jin, You-Xun, Wu, Liqing, Wang, Jing, Sakaguchi, Yohei, Kinumi, Tomoya, Takatsu, Akiko, Kim, Sook-Kyung, Jeong, Ji-Seon
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.09.2019
• Subjects: Certified reference material; Chromatography, High Pressure Liquid - standards; Human growth hormone; Human Growth Hormone - analysis; Human Growth Hormone - chemistry; Humans; International comparison; Mass Spectrometry - standards; Protein quantification; Protein Stability; Recombinant Proteins - analysis; Recombinant Proteins - chemistry; Reference Standards; Stability assessment; Protein quantification; Certified reference material; Human growth hormone; International comparison; Stability assessment

A certified reference material (CRM) for the quantification of protein, essential to manage quality control and quality assurance in protein-related works, has been developed. Amino acid analysis with conventional acid hydrolysis and isotope dilution HPLC-MS was used to establish an SI-traceable abs...