Acetylcholinesterase of mangrove oyster Crassostrea rhizophorae: A highly thermostable enzyme with promising features for estuarine biomonitoring

• Published in: Aquatic toxicology Vol. 197; pp. 109 - 121
• Main Authors: de Souza, Paula Rayane, de Souza, Kelma Sirleide, de Assis, Caio Rodrigo Dias, de Araújo, Marlyete Chagas, Silva, Kaline Catiely Campos, de Fátima Xavier da Silva, Juliett, Ferreira, Andreia Cybelle Marques, da Silva, Valdir Luna, Adam, Mônica Lúcia, de Carvalho, Luiz Bezerra, de Souza Bezerra, Ranilson
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2018
• Subjects: Acetylcholinesterase - metabolism; Animals; Benzoylurea; Biocatalysis - drug effects; Biomonitoring; Carbamate; Cholinesterase; Cholinesterase Inhibitors - toxicity; Crassostrea - enzymology; Environmental Monitoring; Enzyme Stability - drug effects; Estuaries; Hydrogen-Ion Concentration; Inhibitory Concentration 50; Kinetics; Mangrove oyster; Organophosphorus; Pesticides - toxicity; Substrate Specificity - drug effects; Temperature; Water Pollutants, Chemical - toxicity; Organophosphorus; Benzoylurea; Biomonitoring; Cholinesterase; Carbamate; Mangrove oyster

•AChE from gills and viscera of C. rhizophorae were physicochemical and kinetically characterized.•Parameters such as catalytic efficiency, activation energy and rate enhancements related to the enzymes were obtained.•The enzymes were exposed to carbamate, organophosphorus and benzoylurea pesticides...