Micromass culture of human anulus cells: morphology and extracellular matrix production

• Published in: Spine (Philadelphia, Pa. 1976) Vol. 35; no. 10; p. 1033
• Main Authors: Gruber, Helen E, Chow, Yin, Hoelscher, Gretchen L, Ingram, Jane A, Zinchenko, Natalia, Norton, H James, Sun, Yubo, Hanley, Jr, Edward N
• Format: Journal Article
• Language: English
• Published: United States 01.05.2010
• Subjects: Adult; Aged; Cell Culture Techniques; Cell Differentiation - drug effects; Cell Differentiation - physiology; Cell Proliferation - drug effects; Cells, Cultured; Chondrocytes - drug effects; Chondrocytes - metabolism; Chondrocytes - ultrastructure; Culture Media - chemistry; Culture Media - pharmacology; Extracellular Matrix Proteins - drug effects; Extracellular Matrix Proteins - genetics; Extracellular Matrix Proteins - metabolism; Female; Fibrocartilage - drug effects; Fibrocartilage - physiology; Fibrocartilage - ultrastructure; Gene Expression Regulation - drug effects; Gene Expression Regulation - physiology; Humans; Intervertebral Disc - drug effects; Intervertebral Disc - physiology; Intervertebral Disc - ultrastructure; Intervertebral Disc Displacement - surgery; Male; Microscopy, Electron, Transmission; Middle Aged; Peroxisomes - drug effects; Peroxisomes - metabolism; Peroxisomes - ultrastructure; Proteoglycans - analysis; Proteoglycans - metabolism; Tissue Transplantation - methods; Transforming Growth Factor beta - pharmacology

Micromass culture was assessed as a cell culture microenvironment for anulus cells from the human intervertebral disc. To determine whether the micromass culture technique might be useful for the culture of human anulus cells. Culture of cells in micromass has been traditionally used as a method to...