Direct sputum analysis of Pseudomonas aeruginosa macrorestriction fragment genotypes in patients with cystic fibrosis

• Published in: Medical microbiology and immunology Vol. 186; no. 2-3; pp. 93 - 99
• Main Authors: BREITENSTEIN, S, WALTER, S, BOSSHAMMER, J, RÖMLING, U, TÜMMLER, B
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.10.1997
• Subjects: Adolescent; Adult; Bacterial diseases; Bacterial diseases of the respiratory system; Bacteriology; Biological and medical sciences; Chronic Disease; Cystic Fibrosis - complications; Cystic Fibrosis - microbiology; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Electrophoresis, Gel, Pulsed-Field; Female; Fundamental and applied biological sciences. Psychology; Genotype; Human bacterial diseases; Humans; Infectious diseases; Male; Medical sciences; Medicin och hälsovetenskap; Microbiology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - isolation & purification; Pseudomonas Infections - complications; Pseudomonas Infections - microbiology; Respiratory Tract Infections - complications; Respiratory Tract Infections - microbiology; Sputum - microbiology; Pseudomonadales; Human; Typing; Respiratory disease; Metabolic diseases; Genotype; Cystic fibrosis; Genetic disease; Infection; Chronic; Pulsed field electrophoresis; DNA; Sputum; Bacteriosis; Digestive diseases; Bacteria; Pseudomonadaceae; Pseudomonas aeruginosa; Pancreatic disease

The distribution of bacterial populations in the airways of 13 patients with cystic fibrosis who were colonized for 6-23 years with Pseudomonas aeruginosa was investigated by genotyping of bacterial chromosomes directly isolated from 21 sputa. After removal of host material from sputum by hypotonic...