Dietary polysaccharides from guavira pomace, a co-product from the fruit pulp industry, display therapeutic application in gut disorders

• Published in: Food research international Vol. 156; p. 111291
• Main Authors: Mulinari Turin de Oliveira, Natalia, Barbosa da Luz, Bruna, Schneider, Vanessa Suzane, Barbosa da Costa Filho, Humberto, Sérgio de Araujo Sousa, Paulo, Fernanda de Paula Werner, Maria, Henrique Loiola Ponte de Souza, Marcellus, Almeida Rocha, Jefferson, Antonio Duarte Nicolau, Lucas, Mach Côrtes Cordeiro, Lucimara, Maria-Ferreira, Daniele
• Format: Journal Article
• Language: English
• Published: Canada Elsevier Ltd 01.06.2022
• Subjects: Abdominal pain; Abdominal Pain - drug therapy; Animals; Campomanesia sp; Capsaicin; Colitis, Ulcerative - drug therapy; Diarrhea; Dietary Carbohydrates; Fruit; Guavira pomace; Humans; Inflammatory bowel disease; Inflammatory Bowel Diseases; Mice; Molecular Docking Simulation; Natural polysaccharide; Polysaccharides - pharmacology; Inflammatory bowel disease; Natural polysaccharide; Abdominal pain; Campomanesia sp; Guavira pomace
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2022.111291

[Display omitted] •CPW is a dietary polysaccharide from guavira pomace a co-product of the fruit pulp industry.•CPW ameliorates visceral hypersensitivity.•CPW regulates intestinal motility and diarrhea, and reverses intestinal inflammation.•CPW specific units may bind to the 3N8V, 5COX, 2J67, and 6RBF proteins.•CPW has attractive therapeutic characteristics for the treatment of abdominal pain and ulcerative colitis. Inflammatory bowel disease (IBD) includes two distinct diseases: Crohn's disease (CD) and ulcerative colitis (UC). IBD is a chronic systemic disease of the gastrointestinal tract, characterized by an inflammatory process. The mechanisms by which diseases develop are still unknown, but it is known that it results from a complex interaction between genetic variability, the host's immune system, and environmental factors. One of the main complaints of patients is abdominal pain, which may be associated with the release of inflammatory mediators, changes in the normal motility of the digestive tract, and increased intestinal permeability. Currently available drugs for abdominal pain are not satisfactory, therefore, it is extremely necessary to seek new therapeutic options for the treatment of abdominal pain. Polysaccharides extracted from fruits have attracted interest, as these molecules protect the intestinal mucosa and promote wound healing, attenuating inflammation, pain, and altered intestinal motility. In this study, we investigated the ability of pectic polysaccharides obtained from guavira pomace, named CPW to reduce visceral hypersensitivity, regulate intestinal motility, and control diarrhea in mice. Acetic acid, capsaicin, or mustard oil were used to assess visceral pain in normal mice. CPW reduced abdominal writhing, cell migration, and capsaicin-induced visceral nociception. Furthermore, it regulated intestinal motility and all measured parameters of castor oil-induced diarrhea. CPW treatment reversed the increase in mucosal permeability, TEER, and tissue weight caused by acetic acid. In addition, molecular docking analysis showed that specific the CPW units binds to the 3N8V, 5COX, 2J67 and 6RBF proteins. Thus, the results suggest that CPW has attractive therapeutic characteristics for the treatment of abdominal pain and ulcerative colitis.