cytosolic trehalase from the thermophilic fungus Humicola grisea var. thermoidea

• Published in: Microbiology (Society for General Microbiology) Vol. 140; no. 7; pp. 1671 - 1677
• Main Authors: Cardello, L, Terenzi, H.F, Jorge, J.A
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.07.1994; Society for General Microbiology
• Subjects: Biological and medical sciences; characterization; conidia; cytosol; enzyme activity; Fundamental and applied biological sciences. Psychology; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Humicola; Humicola grisea thermoidea; Microbiology; Mycology; physicochemical properties; purification; thermophilic fungi; trehalase; Purification; Enzyme; Enzyme inhibitor; Cytosol; Thermal stability; Fungi; Enzymatic activity; α,α-Trehalase; Glycosidases; Substrate specificity; Hydrolases; Fungi Imperfecti; Kinetic parameter; O-Glycosidases; Physicochemical properties; Thallophyta

A cytosolic trehalase was purified from the thermophilic fungus Humicola grisea var. thermoidea. The apparent molecular mass of the purified native enzyme was estimated to be 360 kDa by gel filtration chromatography. A single polypeptide band of approximate molecular mass 120 kDa was detected by SDS...