Effect of chlorhexidine gluconate on mechanical and anti-microbial properties of thermoplastic cassava starch

• Published in: Carbohydrate polymers Vol. 275; p. 118690
• Main Authors: Thajai, Nanthicha, Jantanasakulwong, Kittisak, Rachtanapun, Pornchai, Jantrawut, Pensak, Kiattipornpithak, Krittameth, Kanthiya, Thidarat, Punyodom, Winita
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2022
• Subjects: Anti-Infective Agents, Local - chemistry; Anti-Infective Agents, Local - pharmacology; Antimicrobial; Aspergillus oryzae - drug effects; Bacillus cereus - drug effects; Biopolymer; Candida albicans - drug effects; Chlorhexidine - analogs & derivatives; Chlorhexidine - chemistry; Chlorhexidine - pharmacology; chlorhexidine gluconate; Escherichia coli - drug effects; Glycerol - chemistry; Magnetic Resonance Spectroscopy - methods; Manihot - chemistry; Manihot esculenta; Solubility; Staphylococcus aureus - drug effects; Starch - chemistry; Temperature; Tensile Strength; Water - chemistry; Antimicrobial; Manihot esculenta; chlorhexidine gluconate; Biopolymer
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2021.118690

Antimicrobial thermoplastic starch (TPS) was developed using cassava starch, glycerol, and chlorhexidine gluconate (CHG) blend. CHG was added at concentrations of 1%, 5%, 10%, and 20% (wt./wt.) as an antimicrobial additive. The tensile strength and hardness of the blended samples increased with the chlorhexidine gluconate content, especially for 1% CHG. wt./wt. (12.6 MPa and 94, respectively). The TPS/CHG20 blend exhibited a large phase of CHG and recrystallization of TPS. The water solubility decreased with the addition of CHG. Nuclear magnetic resonance data confirmed a reaction between the hydroxyl groups of TPS and the amino groups of CHG. The TPS/CHG20% exhibited an inhibition zone for three bacterial types (Staphylococcus aureus, Escherichia coli, and Bacillus cereus) and three fungal types (Rhizopus oligosporus, Aspergillus oryzae, and Candida albicans). CHG acted simultaneously as a chain extender and an antimicrobial additive for TPS, improving its tensile strength, hardness, and anti-microbial properties. •Antimicrobial thermoplastic starch (TPS) using chlorhexidine gluconate (CHG)•CHG acted as a dual chain extender and antimicrobial for TPS.•Mechanical properties of TPS were improved by CHG addition.•NMR confirmed a reaction between TPS and CHG.•The TPS/CHG20% inhibited three bacterium and three fungus types.