Superhydrophobic candle soot/PDMS substrate for one-step enrichment and desalting of peptides in MALDI MS analysis

• Published in: Talanta (Oxford) Vol. 190; pp. 23 - 29
• Main Authors: Wang, Xueyun, Li, Ning, Xu, Daren, Yang, Xiangchao, Zhu, Qunyan, Xiao, Dongyang, Lu, Nan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2018
• Subjects: Amino Acid Sequence; Animals; Cattle; Desalting; Dimethylpolysiloxanes - chemistry; Enrichment; Hydrophobic and Hydrophilic Interactions; MALDI MS; Milk - chemistry; One-step; Peptides - chemistry; Peptides - isolation & purification; Salts - chemistry; Soot - chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Superhydrophobic candle soot/PDMS substrate; Enrichment; One-step; Superhydrophobic candle soot/PDMS substrate; Desalting; MALDI MS

Superhydrophobic substrate is applied in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) detection due to its confinement effect. The weak interaction of superhydrophobic surface with water/salts makes it potential in one-step enrichment and desalting of peptide in MALDI MS...