Molecular characterization of a cDNA encoding vitellogenin in the coonstriped shrimp, Pandalus hypsinotus and site of vitellogenin mRNA expression

• Published in: Journal of experimental zoology. Part A, Comparative experimental biology Vol. 301; no. 10; pp. 802 - 814
• Main Authors: Tsutsui, Naoaki, Saido-Sakanaka, Hisako, Yang, Wei-Jun, Jayasankar, Vidya, Jasmani, Safiah, Okuno, Atsuro, Ohira, Tsuyoshi, Okumura, Takuji, Aida, Katsumi, Wilder, Marcy N
• Format: Journal Article
• Language: English
• Published: United States 01.10.2004
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Cluster Analysis; DNA Primers; DNA, Complementary - genetics; Female; Gene Components; Hepatopancreas - metabolism; Japan; Male; Molecular Sequence Data; Pandalidae - genetics; Pandalus hypsinotus; Phylogeny; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology; Vitellogenins - genetics; Vitellogenins - metabolism; Japan
• DOI: 10.1002/jez.a.53
• ISSN: 1548-8969; 1932-5223

In order to determine the primary structure of vitellogenin in a protandric species, the coonstriped shrimp Pandalus hypsinotus, we previously purified four vitellin components (designated as VnA, VnB, VnC, and VnD, respectively), and chemically analyzed their partial amino acid sequences. In this study, we subsequently cloned a cDNA encoding vitellogenin in this species based on the N-terminal and internal amino acid sequences of VnA, as well as the N-terminal sequence of VnC. The open reading frame of this cDNA encoded a pro-vitellogenin in which vitellins were arranged as follows: NH2-VnA-VnB-VnC/D-COOH. The deduced amino acid sequence possessed a single consensus cleavage sequence, R-X-K/R-R, along the lines of vitellogenins reported in other crustaceans and insects, and the N-terminal sequence of VnB was immediately preceded by this sequence. The comparison of primary structures revealed the existence of a basic and characteristic structure for the vitellogenin molecule in decapod crustacean species, and phylogenetic analysis reflected the current taxonomic classifications of Crustacea. An approximately 8 kb-long transcript of the vitellogenin gene was detected in the hepatopancreas of female shrimps having a gonadosomatic index higher than 1.0 by Northern blot analysis, but was not observed in the hepatopancreas and gonads of male shrimps and the hepatopancreas of female shrimps having a gonadosomatic index lower than 1.0. These results indicate that the hepatopancreas is responsible for vitellogenin synthesis.