Optical tracking and detection of immunomagnetically selected and aligned cells

• Published in: Nature biotechnology Vol. 17; no. 12; pp. 1210 - 1213
• Main Authors: Tibbe, Arjan G.J., Grooth, Bart G. de, Greve, Jan, Liberti, Paul A., Dolan, Gerald J., Terstappen, Leon W.M.M.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.12.1999; Nature
• Subjects: Agriculture; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Computer Simulation; Cytological Techniques; Diverse techniques; Fundamental and applied biological sciences. Psychology; Humans; immunomagnetic selection; Immunomagnetic Separation; Leukocytes - cytology; Life Sciences; Molecular and cellular biology; Optics and Photonics; research-article; Cytometry; Blood cell; Immunomagnetic method; Optical tracking; Analysis method; Leukocyte; Fluorescent labelling; Ferromagnetic materials; Blood

We have developed a platform for cell analysis based on immunomagnetic selection and magnetic alignment of cells in combination with an epi-illumination tracking and detection system. Whole blood was labeled with ferromagnetic nanoparticles and fluorescent probes, and placed in a magnetic field in a...