Development of a new online SPE-HPLC-MS/MS method for the profiling and quantification of sphingolipids and phospholipids in red blood cells – Application to the study of Gaucher's disease

• Published in: Analytica chimica acta Vol. 1278; p. 341719
• Main Authors: Bettioui, Terkia, Chipeaux, Caroline, Ben Arfa, Kaouther, Héron, Sylvie, Belmatoug, Nadia, Franco, Mélanie, de Person, Marine, Moussa, Fathi
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 16.10.2023; Elsevier Masson
• Subjects: Chemical Sciences; Gaucher's disease; Online SPE-HPLC-MS/MS; Red blood cells lipidome; Sphingolipid and phospholipid profiling; Red blood cells lipidome; Online SPE-HPLC-MS/MS; Sphingolipid and phospholipid profiling; Gaucher's disease

Red blood cells (RBCs) are the subject of clinical attention due to their biological importance. Recently, it has been shown that certain erythrocyte pathologies could be linked to an abnormal lipid composition. In this work, we have developed a simple and fast method using online sample preparation...