Rates and impact of human antibody glycation in vivo

• Published in: Glycobiology (Oxford) Vol. 22; no. 2; pp. 221 - 234
• Main Authors: Goetze, Andrew M, Liu, Y Diana, Arroll, Thomas, Chu, Lily, Flynn, Gregory C
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.02.2012
• Subjects: Chromatography, Reverse-Phase; Glucose - chemistry; Glycosylation; Histocompatibility Antigens Class I - chemistry; Histocompatibility Antigens Class I - metabolism; Humans; Hydrogen-Ion Concentration; Immunoglobulin Fc Fragments - chemistry; Immunoglobulin G - chemistry; Immunoglobulin G - metabolism; Mass Spectrometry; Peptide Mapping; Protein Processing, Post-Translational; Receptors, Fc - chemistry; Receptors, Fc - metabolism; Staphylococcal Protein A - chemistry; Staphylococcal Protein A - metabolism; Temperature; peptide mapping; effector function; posttranslational modifications
• ISSN: 0959-6658; 1460-2423; 1460-2423
• DOI: 10.1093/glycob/cwr141

Glycation of immunoglobulin G (IgG) can result from incubation with a reducing sugar in vitro or during circulation in vivo. Upon injection of a recombinantly produced human therapeutic IgG into humans, changes in the glycation levels could be observed as a function of circulation time. Mass changes...