ERA-CRISPR/Cas12a-based, fast and specific diagnostic detection for Chlamydia pneumoniae

• Published in: Frontiers in cellular and infection microbiology Vol. 14; p. 1477422
• Main Authors: Zhou, Yanxia, Yan, Zijun, Zhou, Shi, Li, Weiwei, Yang, Hongyu, Chen, Hongliang, Deng, Zhongliang, Zeng, Qilin, Sun, Peiyuan, Wu, Yimou
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 01.11.2024
• Subjects: Bacterial Proteins - genetics; Chlamydia pneumoniae; Chlamydophila Infections - diagnosis; Chlamydophila pneumoniae - genetics; Chlamydophila pneumoniae - isolation & purification; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR-Associated Proteins - genetics; CRISPR-Cas Systems; CRISPR/Cas12a; Endodeoxyribonucleases; ERA; Humans; Molecular Diagnostic Techniques - methods; Nucleic Acid Amplification Techniques - methods; OmpA gene; pathogenic bacteria detection; Sensitivity and Specificity; trans-cleavage; OmpA gene; Chlamydia pneumoniae; ERA; trans-cleavage; pathogenic bacteria detection; CRISPR/Cas12a

Chlamydia pneumoniae (C. pneumoniae) is a specialized intracellular parasitic pathogen capable of causing pneumonia, sinusitis, bronchitis, and other respiratory diseases, which pose significant public health challenges. Therefore, rapid, accurate, and sensitive diagnosis is crucial for the preventi...