Identification of commercial fish species belonging to the orders pleuronectiformes and gadiformes: library of isoelectric focusing patterns

Isoelectric focusing in polyacrylamide gel was used to establish an identification archive of fish species belonging to the orders Pleuronectiformes, or flat fish, and Gadiformes, or gadoid fish. The 2 orders include species of different commercial value and interest that are frequently requested in...

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Bibliographic Details
Published inJournal of AOAC International Vol. 84; no. 5; p. 1600
Main Authors Tepedino, V, Berrini, A, Borromeo, V, Gaggioli, D, Cantoni, C, Manzoni, P, Secchi, C
Format Journal Article
LanguageEnglish
Published England 01.09.2001
Subjects
Animals
Electrophoresis, Polyacrylamide Gel
Fishes - metabolism
Flatfishes - metabolism
Hydrogen-Ion Concentration
Isoelectric Focusing
Meat - analysis
Species Specificity
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Summary:Isoelectric focusing in polyacrylamide gel was used to establish an identification archive of fish species belonging to the orders Pleuronectiformes, or flat fish, and Gadiformes, or gadoid fish. The 2 orders include species of different commercial value and interest that are frequently requested in European fish markets, but are susceptible to substitution either because they are morphologically similar or because they arrive on the markets already filleted or sliced. The sarcoplasmic protein profiles are species-specific and reproducible. The use of densitometry and image analysis coupled with a simple computer program overcomes the subjective evaluation of the patterns, making it possible to identify species correctly.
ISSN:1060-3271
DOI:10.1093/jaoac/84.5.1600