Molecular, Genetic, and Biochemical Characterization of OXA-484 Carbapenemase, a Difficult-to-Detect R214G Variant of OXA-181

• Published in: Microorganisms (Basel) Vol. 12; no. 7; p. 1391
• Main Authors: Gonzalez, Camille, Oueslati, Saoussen, Rima, Mariam, Nermont, Réva, Dortet, Laurent, Hopkins, Katie L, Iorga, Bogdan I, Bonnin, Rémy A, Naas, Thierry
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.07.2024; MDPI
• Subjects: antibiotic resistance; Antibiotics; Antimicrobial agents; Bacteriology; beta-lactamase; Carbapenemase; Carbapenems; Citrobacter; Clinical isolates; Cloning; detection; E coli; Enzymes; Food; Gene flow; Genes; Genomes; Genomics; Hydrolysis; Imipenem; Immunoassay; Immunoassays; Life Sciences; Microbiology and Parasitology; Molecular modelling; OXA-181; oxacillinase; Plasmids; Selective media; Substrates; Temocillin; United Kingdom > UK; France; United States > US; Germany; oxacillinase; detection; OXA-181; beta-lactamase; carbapenemase; antibiotic resistance

OXA-244, an R214G variant of OXA-48, is silently spreading worldwide likely because of difficulties in detection using classical screening media. Here, we characterized two clinical isolates of and that displayed reduced susceptibility to carbapenems but were lacking significant carbapenemase activi...