Dual-transgenic BiFC vector systems for protein-protein interaction analysis in plants

• Published in: Frontiers in genetics Vol. 15; p. 1355568
• Main Authors: Chen, Piaojuan, Ye, Meiling, Chen, Yadi, Wang, Qin, Wang, Qiongli, Zhong, Ming
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 08.03.2024
• Subjects: bimolecular fluorescence complementation (BiFC); EYFP; mCherry; mRFP1Q66T; mVenus; protein-protein interaction (PPI); protein-protein interaction (PPI); mCherry; mVenus; bimolecular fluorescence complementation (BiFC); mRFP1Q66T; EYFP

Protein-protein interaction (PPI) play a pivotal role in cellular signal transduction. The bimolecular fluorescence complementation (BiFC) assay offers a rapid and intuitive means to ascertain the localization and interactions of target proteins within living cells. BiFC is based on fluorescence com...