OMIP 071: A 31‐Parameter Flow Cytometry Panel for In‐Depth Immunophenotyping of Human T‐Cell Subsets Using Surface Markers

• Published in: Cytometry. Part A Vol. 99; no. 3; pp. 273 - 277
• Main Authors: Wang, Song‐Rong, Zhong, Na, Zhang, Xin‐Mei, Zhao, Zhi‐Bin, Balderas, Robert, Li, Liang, Lian, Zhe‐Xiong
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.03.2021; Wiley Subscription Services, Inc
• Subjects: CC chemokine receptors; CCR6 protein; CCR7 protein; CD103 antigen; CD28 antigen; CD57 antigen; CD69 antigen; CD7 antigen; CD95 antigen; Cell activation; Cell differentiation; Cell surface; Chemokine receptors; Chemokines; CXCR3 protein; CXCR4 protein; CXCR5 protein; Differentiation (biology); Flow cytometry; Helper cells; Histocompatibility antigen HLA; human T‐cell subsets cell surface staining31‐parameter flow cytometry panelPBMCs; Lymph nodes; Lymphocytes; Lymphocytes T; Markers; Parameters; Peripheral blood; Receptors; Surface markers; T cell receptors; Tissue analysis; human T-cell subsets cell surface staining31-parameter flow cytometry panelPBMCs

Dissecting the functional diversity of T cells is critical in elucidating mechanisms and in developing therapies for various diseases. Here, we designed a 31‐parameter (29‐color) panel to enable the characterization of T‐cell subsets and immunophenotyping of the human peripheral blood and lymph node...