AtoSC two-component system is involved in cPHB biosynthesis through fatty acid metabolism in E. coli

• Published in: Biochimica et biophysica acta Vol. 1810; no. 5; pp. 561 - 568
• Main Authors: Theodorou, Evaggelos C, Theodorou, Marina C, Kyriakidis, Dimitrios A
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2011
• Subjects: Acetoacetates - pharmacology; Acrylates - pharmacology; acrylic acid; beta oxidation; biochemical pathways; biosynthesis; cerulenin; Cerulenin - pharmacology; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; fatty acid metabolism; Fatty Acid Synthesis Inhibitors - pharmacology; fatty acids; Fatty Acids - metabolism; gene expression; Hydroxybutyrates - metabolism; Immunoblotting; Models, Biological; operon; Oxidation-Reduction - drug effects; plasmids; Plasmids - genetics; Polyesters - metabolism; Protein Kinases - genetics; Protein Kinases - metabolism; spermidine; Spermidine - pharmacology; Time Factors

BACKGROUND: We have shown previously that AtoSC two-component system regulates the biosynthesis of E. coli cPHB [complexed poly-(R)-3-hydroxybutyrate]. METHODS: The AtoSC involvement on fatty acids metabolism, towards cPHB synthesis, was studied using cPHB determination, gene expression, and fatty a...