Isothermal cross-boosting extension-nicking reaction mediated exponential signal amplification for ultrasensitive detection of polynucleotide kinase

• Published in: Analyst (London) Vol. 145; no. 1; pp. 3742 - 3748
• Main Authors: Li, Xiao-Yu, Cui, Yun-Xi, Du, Yi-Chen, Tang, An-Na, Kong, De-Ming
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 21.05.2020
• Subjects: Adenine; Amplification; Binding sites; Biosensing Techniques - methods; DNA Probes - genetics; DNA Probes - metabolism; Elongation; Enlargement; Fluorescence; HeLa Cells; Humans; Kinases; Limit of Detection; Nucleic Acid Amplification Techniques; Oligonucleotides; Polynucleotide 5'-Hydroxyl-Kinase - metabolism; Substrates; Uracil

A novel nucleic acid-based isothermal signal amplification strategy, named cross-boosting extension-nicking reaction (CBENR) is developed and successfully used for rapid and ultrasensitive detection of polynucleotide kinase (PNK) activity. Only two simple oligonucleotides (recognition substrate ( RS...