Inhibition of cathepsin proteases attenuates migration and sensitizes aggressive N-Myc amplified human neuroblastoma cells to doxorubicin

• Published in: Oncotarget Vol. 6; no. 13; pp. 11175 - 11190
• Main Authors: Gangoda, Lahiru, Keerthikumar, Shivakumar, Fonseka, Pamali, Edgington, Laura E, Ang, Ching-Seng, Ozcitti, Cemil, Bogyo, Matthew, Parker, Belinda S, Mathivanan, Suresh
• Format: Journal Article
• Language: English
• Published: United States Impact Journals LLC 10.05.2015
• Subjects: Antibiotics, Antineoplastic - pharmacology; Apoptosis; Blotting, Western; Cathepsins - antagonists & inhibitors; Cell Movement; Cell Proliferation; Chromatography, Liquid; Doxorubicin - pharmacology; Drug Resistance, Neoplasm; Gene Expression Regulation, Enzymologic - drug effects; Gene Expression Regulation, Neoplastic - drug effects; Humans; Neuroblastoma - drug therapy; Neuroblastoma - metabolism; Neuroblastoma - pathology; Protease Inhibitors - pharmacology; Proteomics; Proto-Oncogene Proteins c-myc - genetics; Proto-Oncogene Proteins c-myc - metabolism; Real-Time Polymerase Chain Reaction; Research Paper; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Tandem Mass Spectrometry; Tumor Cells, Cultured; Wound Healing; neuroblastoma; mass spectrometry; proteomics; N-Myc amplification; secretome

Neuroblastoma arises from the sympathetic nervous system and accounts for 15% of childhood cancer mortality. Amplification of the oncogene N-Myc is reported to occur in more than 20% of patients. While N-Myc amplification status strongly correlates with higher tumour aggression and resistance to tre...