Chalcone synthase cosuppression phenotypes in petunia flowers: comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences

• Published in: Plant molecular biology Vol. 31; no. 5; p. 957
• Main Authors: Jorgensen, R.A. (California Univ., Davis, CA (USA). Dept. of Environmental Horticulture), Cluster, P.D, English, J, Que, Q, Napoli, C.A
• Format: Journal Article
• Language: English
• Published: Netherlands 01.08.1996
• Subjects: Acyltransferases - genetics; CALCONA; CHALCONE; CHALCONES; CODE GENETIQUE; CODIGO GENETICO; Crosses, Genetic; DNA, Antisense; DNA, Bacterial; FENOTIPOS; FLEUR; FLORES; FLOWERS; Gene Dosage; Gene Expression Regulation, Plant; GENETIC CODE; NUCLEOTIDE SEQUENCE; PETUNIA; PHENOTYPE; PHENOTYPES; PIGMENTACION; PIGMENTATION; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants, Genetically Modified - enzymology; Plants, Genetically Modified - genetics; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; Suppression, Genetic; TRANSGENIC PLANTS

Flower pigmentation patterns were scored in 185 sense Chalcone synthase (Chs) transgenotes and 85 antisense Chs transgenotes; upon first flowering, 139 (75%) of sense transgenotes were found to be phenotypically altered, as were 70 (82%) of the antisense transgenotes. The observed patterns document...