Simultaneous Determination of Carvedilol, Enalaprilat, and Perindoprilat in Human Plasma Using LC–MS/MS and Its Application to a Pharmacokinetic Pilot Study

• Published in: Chromatographia Vol. 85; no. 5; pp. 455 - 468
• Main Authors: Joubert, Andre, Kellermann, Tracy, Joubert, Anton, van der Merwe, Marthinus, Norman, Jennifer, Castel, Sandra, Sliwa, Karen, Maartens, Gary, Sinxadi, Phumla, Wiesner, Lubbe
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2022; Springer Nature B.V
• Subjects: Analytical Chemistry; Blood plasma; Calibration; Chemistry; Chemistry and Materials Science; Chromatography; Coefficient of variation; High performance liquid chromatography; Human performance; Ions; Laboratory Medicine; Mass spectrometry; Mathematical analysis; Original; Pharmacokinetics; Pharmacology; Pharmacy; Proteomics; Recovery; Selectivity; Stability analysis; Adherence; Perindoprilat; LC–MS/MS; Carvedilol; Heart failure (HF); Enalaprilat

A method for the extraction and quantification of carvedilol, enalaprilat, and perindoprilat in 50 µL human plasma, using high-performance liquid chromatography with tandem mass spectrometry (LC–MS/MS) detection was developed and validated. Samples were prepared via protein precipitation with chroma...