Protective effect of polysaccharides from Sargassum fusiforme against UVB-induced oxidative stress in HaCaT human keratinocytes

• Published in: Journal of functional foods Vol. 36; pp. 332 - 340
• Main Authors: Ji, Desheng, You, Lijun, Ren, Yinglang, Wen, Lingrong, Zheng, Guiqing, Li, Chao
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.09.2017; Elsevier
• Subjects: HaCaT keratinocytes; Matrix metalloproteinase (MMPs); Oxidative stress; Sargassum fusiforme polysaccharides; UVB; HaCaT keratinocytes; UVB; Oxidative stress; Matrix metalloproteinase (MMPs); Sargassum fusiforme polysaccharides
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2017.06.051

•A polysaccharide fraction (SFP-P1) was purified from Sargassum fusiform.•The composition and structural characteristics of SFP-P1 were investigated.•SFP-P1 increased the activities of SOD, GSH-PX and decreased the level of ROS.•SFP-P1 suppressed the UVB-induced expression of MMP-1 and MMP-9. In this study, Sargassum fusiforme polysaccharide was purified using a DEAE-Sepharose fast-flow column to obtain Sargassum fusiforme polysaccharides fraction (SFP-P1). As expected, the SFP-P1 contains mainly carbohydrates (87%) with less than 1% protein. Within the carbohydrate, it contains about 10% sulfate and 18% uronic acids. SFP-P1 consisted of l-fucose (13.17%), galactose (4.28%), glucose (1.95%), xylose (5.50%), and mannose (75.10%) with an average molecular weight of 113kDa. Mannose and l-fucose were two predominant monosaccharides. In addition, (1→)-linked or (1→6)-linked, (1→2)-linked or (1→4)-linked and (1→3)-linked glycosyl linkages accounted for 1.62%, 60.69% and 37.69% of all linkages in the molecule, respectively. The glycosidic linkage types of SFP-P1 were proven to be→3,6)-α-d-Manp(1→,→4)-α-d-GalAp(1→,→4)-β-d-Xylp(1→and→3,4)-α-d-GlcAp(1→. The results indicated the SFP-P1 had cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through stimulation of SOD, GSH-PX enzymes activities and ROS. Also SFP-P1 suppressed the UVB-induced expression of MMP-1 & MMP-9.