Mass spectrometric quantification of microRNAs in biological samples based on multistage signal amplification

• Published in: Analyst (London) Vol. 145; no. 5; pp. 1783 - 1788
• Main Authors: Li, Xiangtang, Zhao, Jingjin, Xu, Rui, Pan, Li, Liu, Yi-Ming
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 07.03.2020
• Subjects: Amplification; Biological properties; Cost analysis; Ions; Liquid chromatography; Mass spectrometry; MicroRNAs; Multistage; Nuclease

This work describes a novel method for quantification of miRNAs based on multistage signal amplification (MSA) and liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). The multistage signal amplification involves hybridization enrichment of miRNA targets with a DNA...