Dynamic changes in the level of WT1 as an MRD marker to predict the therapeutic outcome of patients with AML with and without allogeneic stem cell transplantation

• Published in: Molecular medicine reports Vol. 20; no. 3; pp. 2426 - 2432
• Main Authors: Liu, Huasheng, Wang, Xiaoning, Zhang, Hailing, Wang, Jincheng, Chen, Ying, Ma, Tiantian, Shi, Jing, Kang, Ya, Xi, Jieying, Wang, Mengchang, Zhang, Mei
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications UK Ltd 01.09.2019
• Subjects: Acute myeloid leukemia; Adolescent; Adult; Aged; Apoptosis; Blood cancer; Bone marrow; Chemotherapy; Child; Deoxyribonucleic acid; DNA; Female; Gene Expression Regulation, Leukemic; Genes; Hematology; Hematopoietic Stem Cell Transplantation; Hematopoietic stem cells; Humans; Leukemia; Leukemia, Myeloid, Acute - diagnosis; Leukemia, Myeloid, Acute - genetics; Leukemia, Myeloid, Acute - therapy; Male; Medical prognosis; Middle Aged; Mutation; Myeloid leukemia; Neoplasm, Residual; Pharmaceuticals; Prognosis; Promyeloid leukemia; Remission; Response rates; Reverse transcription; Runx1 protein; Stem cell transplantation; Stem cells; Studies; Transcription factors; Treatment Outcome; Up-Regulation; WT1 Proteins - genetics; Young Adult
• ISSN: 1791-2997; 1791-3004; 1791-3004
• DOI: 10.3892/mmr.2019.10440

Monitoring minimal residue disease (MRD) is an effective approach to evaluate the response to chemotherapy, and it is used to select the ideal therapeutic strategy and to predict the recurrence during therapy for hematological disorders. The Wilm's tumor 1 (WT1) gene, which is highly expressed in >80% of patients with acute myeloid leukemia (AML) and its increased expression level may cause poor clinical outcomes, is a potential MRD marker of hematological neoplasms. In the present study, the expression levels of WT1 and other molecular markers were retrospectively analyzed by reverse transcription‑quantitative PCR in 195 patients with AML. The expression level of WT1 was significantly lower in patients with remission compared with patients with early‑stage and recurrent AML. Moreover, WT1 expression was significantly decreased in patients with RUNX family transcription factor 1‑RUNX1 translocation partner 1 fusion, but higher in patients with promyelocytic leukemia‑retinoic acid receptor α fusion. WT1 expression was significantly reduced during remission. In patients with AML who underwent allogeneic hematopoietic stem cell transplantation (allo‑HSCT), the mortality rate 2 years after allo‑HSCT was significantly lower in patients with low expression level of WT1 compared with subjects presenting high expression level of WT1. Collectively, the upregulation of the expression level of WT1 in combination with the identification of other genetic abnormalities may be used as MRD markers of hematological neoplasms.