Development of an electrochemical‐surface plasmon dual biosensor based on carboxylated conducting polymer thin films

• Published in: Journal of applied polymer science Vol. 135; no. 1
• Main Authors: Sriwichai, Saengrawee, Janmanee, Rapiphun, Phanichphant, Sukon, Shinbo, Kazunari, Kato, Keizo, Kaneko, Futao, Yamamoto, Tadashi, Baba, Akira
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc 05.01.2018
• Subjects: Atomic force microscopy; Biomolecules; biosensor; Biosensors; conducting polymer; Conducting polymers; electrochemical‐surface plasmon resonance spectroscopy; Glucose; human immunoglobulin G; Materials science; Polymer films; Polymers; Scanning electron microscopy; Thin films
• ISSN: 0021-8995; 1097-4628
• DOI: 10.1002/app.45641

ABSTRACT A biosensing platform based on the covalent attachment of biomolecules on electropolymerized carboxylated conducting polymers, poly(3‐aminobenzoic acid) and poly(3‐pyrrole carboxylic acid), were developed for the selective simultaneous detection of two biomolecules using electrochemical‐surface plasmon resonance (EC–SPR) spectroscopy. The surface morphology of the developed biosensors was studied by scanning electron microscopy and atomic force microscopy. The EC–SPR dual biosensor was developed for the label‐free, simultaneous, and selective detection of glucose and human immunoglobulin G (IgG). A change in current density was clearly observed after the injection of glucose, whereas a change in SPR reflectivity was clearly observed after the injection of human IgG. The present work demonstrates the potential of this biosensing platform for real sample analysis in the future. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 45641.