Determination of sequence and absolute configuration of peptide amino acids by HPLC–MS/CD-based detection of liberated N-terminus phenylthiohydantoin amino acids

• Published in: Scientific reports Vol. 12; no. 1; p. 10285
• Main Authors: Hahn, Dongyup, Wang, Weihong, Choi, Hyukjae, Kang, Heonjoong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.06.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/45/611; 639/638/11/296; 639/638/11/872; 639/638/11/873; 639/638/403/935; 639/638/403/936; 639/638/45/611; Absolute configuration; Amino acids; Edman degradation; High-performance liquid chromatography; Humanities and Social Sciences; Hydrolysates; Liquid chromatography; multidisciplinary; N-Terminus; Parathyroid hormone; Peptides; Science; Science (multidisciplinary)

We report a method for the simultaneous determination of the sequence and absolute configuration of peptide amino acids using a combination of Edman degradation and HPLC–MS/CD. Phenylthiohydantoin (PTH) derivatives of 20 pairs of standard d - and l -amino acids were synthesized by the Edman reaction...