Regulation of thrombin activity by ligand-induced topological alteration in a thrombin-binding aptamer

• Published in: Chemical communications (Cambridge, England) Vol. 59; no. 57; pp. 8862 - 8865
• Main Authors: Sasaki, Shogo, Ma, Yue, Hirokawa, Takatsugu, Ikebukuro, Kazunori, Tera, Masayuki, Nagasawa, Kazuo
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 13.07.2023
• Subjects: Binding; Ligands; Thrombin; Topology

Thrombin-binding aptamer (TBA), which forms a G-quadruplex (G4) structure with anti-parallel topology, interacts with thrombin to inhibit its enzymatic activity. Here we show that the G4-topology-altering ligand L2H2-2M2EA- 6LCO ( 6LCO ) changes the anti-parallel topology of TBA G4 to the parallel t...