Regulation of thrombin activity by ligand-induced topological alteration in a thrombin-binding aptamer

Thrombin-binding aptamer (TBA), which forms a G-quadruplex (G4) structure with anti-parallel topology, interacts with thrombin to inhibit its enzymatic activity. Here we show that the G4-topology-altering ligand L2H2-2M2EA- 6LCO ( 6LCO ) changes the anti-parallel topology of TBA G4 to the parallel t...

Full description

Saved in:
Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 59; no. 57; pp. 8862 - 8865
Main Authors Sasaki, Shogo, Ma, Yue, Hirokawa, Takatsugu, Ikebukuro, Kazunori, Tera, Masayuki, Nagasawa, Kazuo
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 13.07.2023
Subjects
Binding
Ligands
Thrombin
Topology
Online AccessGet full text

Cover

Abstract Thrombin-binding aptamer (TBA), which forms a G-quadruplex (G4) structure with anti-parallel topology, interacts with thrombin to inhibit its enzymatic activity. Here we show that the G4-topology-altering ligand L2H2-2M2EA- 6LCO ( 6LCO ) changes the anti-parallel topology of TBA G4 to the parallel topology, thereby abrogating the thrombin-inhibitory activity of TBA. This finding suggests that G4 ligands that alter topology may be promising drug candidates for diseases involving G4-binding proteins. The function of the thrombin-binding aptamer was regulated by the G-quadruplex topology-altering ligand of L2H2-2M2EA- 6LCO , thereby controlling thrombin activity.
AbstractList Thrombin-binding aptamer (TBA), which forms a G-quadruplex (G4) structure with anti-parallel topology, interacts with thrombin to inhibit its enzymatic activity. Here we show that the G4-topology-altering ligand L2H2-2M2EA- 6LCO ( 6LCO ) changes the anti-parallel topology of TBA G4 to the parallel topology, thereby abrogating the thrombin-inhibitory activity of TBA. This finding suggests that G4 ligands that alter topology may be promising drug candidates for diseases involving G4-binding proteins. The function of the thrombin-binding aptamer was regulated by the G-quadruplex topology-altering ligand of L2H2-2M2EA- 6LCO , thereby controlling thrombin activity.
Thrombin-binding aptamer (TBA), which forms a G-quadruplex (G4) structure with anti-parallel topology, interacts with thrombin to inhibit its enzymatic activity. Here we show that the G4-topology-altering ligand L2H2-2M2EA-6LCO (6LCO) changes the anti-parallel topology of TBA G4 to the parallel topology, thereby abrogating the thrombin-inhibitory activity of TBA. This finding suggests that G4 ligands that alter topology may be promising drug candidates for diseases involving G4-binding proteins.
Thrombin-binding aptamer (TBA), which forms a G-quadruplex (G4) structure with anti-parallel topology, interacts with thrombin to inhibit its enzymatic activity. Here we show that the G4-topology-altering ligand L2H2-2M2EA-6LCO (6LCO) changes the anti-parallel topology of TBA G4 to the parallel topology, thereby abrogating the thrombin-inhibitory activity of TBA. This finding suggests that G4 ligands that alter topology may be promising drug candidates for diseases involving G4-binding proteins.Thrombin-binding aptamer (TBA), which forms a G-quadruplex (G4) structure with anti-parallel topology, interacts with thrombin to inhibit its enzymatic activity. Here we show that the G4-topology-altering ligand L2H2-2M2EA-6LCO (6LCO) changes the anti-parallel topology of TBA G4 to the parallel topology, thereby abrogating the thrombin-inhibitory activity of TBA. This finding suggests that G4 ligands that alter topology may be promising drug candidates for diseases involving G4-binding proteins.
Author Ma, Yue
Ikebukuro, Kazunori
Sasaki, Shogo
Tera, Masayuki
Nagasawa, Kazuo
Hirokawa, Takatsugu
AuthorAffiliation Institute of Biomaterials and Bioengineering
Department of Biotechnology and Life Science
Tokyo University of Agriculture and Technology
Tokyo Medical and Dental University
Faculty of Medicine
University of Tsukuba
Transborder Medical Research Center
Research Core Center
Division of Biomedical Science
AuthorAffiliation_xml – sequence: 0
  name: Tokyo Medical and Dental University
– sequence: 0
  name: Faculty of Medicine
– sequence: 0
  name: Transborder Medical Research Center
– sequence: 0
  name: Department of Biotechnology and Life Science
– sequence: 0
  name: University of Tsukuba
– sequence: 0
  name: Institute of Biomaterials and Bioengineering
– sequence: 0
  name: Research Core Center
– sequence: 0
  name: Division of Biomedical Science
– sequence: 0
  name: Tokyo University of Agriculture and Technology
Author_xml – sequence: 1
  givenname: Shogo
  surname: Sasaki
  fullname: Sasaki, Shogo
– sequence: 2
  givenname: Yue
  surname: Ma
  fullname: Ma, Yue
– sequence: 3
  givenname: Takatsugu
  surname: Hirokawa
  fullname: Hirokawa, Takatsugu
– sequence: 4
  givenname: Kazunori
  surname: Ikebukuro
  fullname: Ikebukuro, Kazunori
– sequence: 5
  givenname: Masayuki
  surname: Tera
  fullname: Tera, Masayuki
– sequence: 6
  givenname: Kazuo
  surname: Nagasawa
  fullname: Nagasawa, Kazuo
BackLink https://www.ncbi.nlm.nih.gov/pubmed/37377065$$D View this record in MEDLINE/PubMed
BookMark eNpd0UtLxDAUBeAgio_RjXul4EaEatrbJp2ljE8QBFFwV9LkpkbaZExSYf69HUdH8EJIFt8JIWePbFpnkZDDjJ5nFKYXCqSkOdCq3SC7GbAiLYvqdXN5Lqcph6LcIXshvNNxsrLaJjvAgXPKyl2in7AdOhGNs4nTSXzzrm-MTYSM5tPERdIsks60wqrUWDVIVEl0c9e51kjRJaKL6FfpZWidT8eljG0TMY-iR79PtrToAh787BPycnP9PLtLHx5v72eXD6kElsdUN02moFKZVhJ4qQtQOpcV1XmjS2SaaRQwmmaaA5dNQVmlBDKBCqtC5AgTcrq6d-7dx4Ah1r0JErtOWHRDqPMKKOM5nfKRnvyj727wdnzdUjEoOIwzIcc_amh6VPXcm174Rf37gyM4WwHpXQge9ZpktF7WU1_BbPZdz-2Ij1bYB7l2f_XBFwsWjZE
Cites_doi 10.1021/ja064536h
10.1073/pnas.1909047116
10.1016/j.bbrc.2019.12.103
10.1002/anie.201705724
10.1093/nar/gkn1076
10.1021/acs.bioconjchem.6b00124
10.1038/nchembio864
10.1093/nar/gks068
10.1093/nar/gkn511
10.1021/ja305734x
10.1073/pnas.1422605112
10.1038/355564a0
10.3390/ph11030080
10.1038/nature755
10.1021/ja200786q
10.1002/ejoc.201900692
10.1002/chem.201802691
10.1016/j.ijbiomac.2022.10.128
10.1038/s41598-019-44736-9
10.1002/anie.200801235
10.1016/S0021-9258(17)46749-4
10.1093/nar/gkz1138
10.1089/154545703322860825
10.1016/j.bbagen.2017.01.006
10.1126/science.1175653
10.1021/acschembio.1c00904
10.1039/D2SC01196D
10.1111/cas.14540
10.1016/j.pharmthera.2020.107649
10.1093/nar/gku833
10.1002/ange.201105193
10.1073/pnas.1321436111
10.1073/pnas.90.8.3745
10.1002/tcr.201300015
10.1093/nar/gni134
10.1093/nar/gkl348
10.1074/jbc.M109.018028
10.1093/nar/gks512
10.1093/nar/gkl1057
10.3390/molecules25163686
10.1002/anie.200300589
10.1039/D1CP04718C
10.1111/j.1749-6632.1981.tb29757.x
10.1093/nar/28.9.1969
10.1016/j.ymeth.2006.10.004
10.1016/j.biochi.2015.06.002
10.1021/ja0603958
10.1039/D0RA09413G
10.1074/jbc.M110.101923
10.1073/pnas.182256799
10.1016/0969-2126(93)90015-9
10.1111/j.1749-6632.1986.tb34585.x
10.1007/s00249-010-0587-x
10.1039/D1CC03294A
ContentType Journal Article
Copyright Copyright Royal Society of Chemistry 2023
Copyright_xml – notice: Copyright Royal Society of Chemistry 2023
DBID AAYXX
CITATION
NPM
7SR
7U5
8BQ
8FD
JG9
L7M
7X8
DOI 10.1039/d3cc02308g
DatabaseName CrossRef
PubMed
Engineered Materials Abstracts
Solid State and Superconductivity Abstracts
METADEX
Technology Research Database
Materials Research Database
Advanced Technologies Database with Aerospace
MEDLINE - Academic
DatabaseTitle CrossRef
PubMed
Materials Research Database
Engineered Materials Abstracts
Solid State and Superconductivity Abstracts
Technology Research Database
Advanced Technologies Database with Aerospace
METADEX
MEDLINE - Academic
DatabaseTitleList
CrossRef
MEDLINE - Academic
Materials Research Database
PubMed
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
EISSN 1364-548X
EndPage 8865
ExternalDocumentID 37377065
10_1039_D3CC02308G
d3cc02308g
Genre Journal Article
GroupedDBID ---
-DZ
-JG
-~X
0-7
0R~
29B
4.4
53G
5GY
6J9
705
70~
7~J
AAEMU
AAHBH
AAIWI
AAJAE
AAMEH
AANOJ
AAWGC
AAXHV
AAXPP
ABASK
ABDVN
ABEMK
ABJNI
ABPDG
ABRYZ
ABXOH
ACBEA
ACGFO
ACGFS
ACIWK
ACLDK
ACNCT
ADMRA
ADSRN
AEFDR
AENEX
AENGV
AESAV
AETIL
AFLYV
AFOGI
AFRDS
AFVBQ
AGEGJ
AGKEF
AGRSR
AGSTE
AHGCF
ALMA_UNASSIGNED_HOLDINGS
ANUXI
APEMP
ASKNT
AUDPV
AZFZN
BLAPV
BSQNT
C6K
CS3
DU5
EBS
ECGLT
EE0
EF-
F5P
GGIMP
GNO
H13
HZ~
H~N
IDZ
IH2
J3I
M4U
N9A
O9-
P2P
R7B
R7C
R7D
RAOCF
RCNCU
RPMJG
RRA
RRC
RSCEA
SJN
SKA
SKF
SKH
SLH
TN5
TWZ
UPT
VH6
VQA
WH7
X7L
AAYXX
AFRZK
AKMSF
ALUYA
CITATION
R56
NPM
7SR
7U5
8BQ
8FD
JG9
L7M
7X8
ID FETCH-LOGICAL-c362t-fbb1d38d1fdc375f43df2c80f2bf5e6f6fea3bb1b9237cb4068dae6aede84a2e3
ISSN 1359-7345
1364-548X
IngestDate Fri Jul 11 11:26:02 EDT 2025
Mon Jun 30 03:34:14 EDT 2025
Thu Apr 03 07:03:03 EDT 2025
Tue Jul 01 04:23:06 EDT 2025
Tue Dec 17 20:58:55 EST 2024
IsPeerReviewed true
IsScholarly true
Issue 57
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c362t-fbb1d38d1fdc375f43df2c80f2bf5e6f6fea3bb1b9237cb4068dae6aede84a2e3
Notes https://doi.org/10.1039/d3cc02308g
Electronic supplementary information (ESI) available. See DOI
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ORCID 0000-0002-0437-948X
0000-0003-4430-579X
PMID 37377065
PQID 2836347333
PQPubID 2047502
PageCount 4
ParticipantIDs rsc_primary_d3cc02308g
pubmed_primary_37377065
proquest_miscellaneous_2830672097
crossref_primary_10_1039_D3CC02308G
proquest_journals_2836347333
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 2023-07-13
PublicationDateYYYYMMDD 2023-07-13
PublicationDate_xml – month: 07
  year: 2023
  text: 2023-07-13
  day: 13
PublicationDecade 2020
PublicationPlace England
PublicationPlace_xml – name: England
– name: Cambridge
PublicationTitle Chemical communications (Cambridge, England)
PublicationTitleAlternate Chem Commun (Camb)
PublicationYear 2023
Publisher Royal Society of Chemistry
Publisher_xml – name: Royal Society of Chemistry
References Riccardi (D3CC02308G/cit30/1) 2021; 217
Biswas (D3CC02308G/cit41/1) 2022; 24
Ishikawa (D3CC02308G/cit55/1) 2021; 57
Zhao (D3CC02308G/cit42/1) 2014; 42
Krauss (D3CC02308G/cit29/1) 2012; 40
Tu (D3CC02308G/cit40/1) 2022; 17
González (D3CC02308G/cit11/1) 2009; 284
Shuman (D3CC02308G/cit27/1) 1986; 485
De Cian (D3CC02308G/cit49/1) 2007; 42
Kumari (D3CC02308G/cit9/1) 2007; 3
Siddiqui-Jain (D3CC02308G/cit10/1) 2002; 99
O'Hagan (D3CC02308G/cit38/1) 2019
Macaya (D3CC02308G/cit28/1) 1993; 90
Roach (D3CC02308G/cit21/1) 2020; 48
Wang (D3CC02308G/cit17/1) 1993; 1
Parkinson (D3CC02308G/cit20/1) 2002; 417
Sasaki (D3CC02308G/cit45/1) 2020; 10
Asamitsu (D3CC02308G/cit37/1) 2019; 25
Padmanabhan (D3CC02308G/cit31/1) 1993; 268
Tang (D3CC02308G/cit46/1) 2006; 128
Yu (D3CC02308G/cit51/1) 2006; 128
Gueddouda (D3CC02308G/cit23/1) 2017; 1861
Masai (D3CC02308G/cit22/1) 2019; 9
McConnell (D3CC02308G/cit35/1) 2016; 27
Bugaut (D3CC02308G/cit2/1) 2012; 40
Wu (D3CC02308G/cit12/1) 2019; 116
Belleperche (D3CC02308G/cit34/1) 2018; 11
Ray (D3CC02308G/cit16/1) 2014; 111
Huppert (D3CC02308G/cit4/1) 2008; 36
Mergny (D3CC02308G/cit47/1) 2005; 33
Cogoi (D3CC02308G/cit13/1) 2010; 285
Krüger (D3CC02308G/cit14/1) 2010; 39
Tera (D3CC02308G/cit43/1) 2008; 47
Ambrus (D3CC02308G/cit19/1) 2006; 34
Fenton (D3CC02308G/cit26/1) 1981; 370
Huppert (D3CC02308G/cit5/1) 2007; 35
Iida (D3CC02308G/cit44/1) 2013; 13
Biffi (D3CC02308G/cit15/1) 2012; 134
Heddi (D3CC02308G/cit24/1) 2015; 112
Verma (D3CC02308G/cit3/1) 2009; 37
Heddi (D3CC02308G/cit18/1) 2011; 133
De Rache (D3CC02308G/cit50/1) 2015; 115
Padmanabhan (D3CC02308G/cit32/1) 1996; 52
Mergny (D3CC02308G/cit52/1) 2003; 13
Seimiya (D3CC02308G/cit6/1) 2020; 111
Bryan (D3CC02308G/cit7/1) 2020; 25
Ma (D3CC02308G/cit36/1) 2020; 531
Napolitano (D3CC02308G/cit39/1) 2023; 224
Davis (D3CC02308G/cit1/1) 2004; 43
Bock (D3CC02308G/cit25/1) 1992; 355
Cahoon (D3CC02308G/cit8/1) 2009; 325
Engelhard (D3CC02308G/cit33/1) 2017; 56
Marathias (D3CC02308G/cit53/1) 2000; 28
Karsisiotis (D3CC02308G/cit48/1) 2011; 123
Zhu (D3CC02308G/cit54/1) 2022; 13
References_xml – volume: 128
  start-page: 15461
  year: 2006
  ident: D3CC02308G/cit51/1
  publication-title: J. Am. Chem. Soc.
  doi: 10.1021/ja064536h
– volume: 116
  start-page: 20453
  year: 2019
  ident: D3CC02308G/cit12/1
  publication-title: Proc. Natl. Acad. Sci. U. S. A.
  doi: 10.1073/pnas.1909047116
– volume: 531
  start-page: 3
  year: 2020
  ident: D3CC02308G/cit36/1
  publication-title: Biochem. Biophys. Res. Commun.
  doi: 10.1016/j.bbrc.2019.12.103
– volume: 56
  start-page: 11640
  year: 2017
  ident: D3CC02308G/cit33/1
  publication-title: Angew. Chem., Int. Ed.
  doi: 10.1002/anie.201705724
– volume: 52
  start-page: 272
  year: 1996
  ident: D3CC02308G/cit32/1
  publication-title: Acta Crystallogr.
– volume: 37
  start-page: 4194
  year: 2009
  ident: D3CC02308G/cit3/1
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkn1076
– volume: 27
  start-page: 1493
  year: 2016
  ident: D3CC02308G/cit35/1
  publication-title: Bioconjugate Chem.
  doi: 10.1021/acs.bioconjchem.6b00124
– volume: 3
  start-page: 218
  year: 2007
  ident: D3CC02308G/cit9/1
  publication-title: Nat. Chem. Biol.
  doi: 10.1038/nchembio864
– volume: 40
  start-page: 4727
  year: 2012
  ident: D3CC02308G/cit2/1
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gks068
– volume: 36
  start-page: 6260
  year: 2008
  ident: D3CC02308G/cit4/1
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkn511
– volume: 134
  start-page: 11974
  year: 2012
  ident: D3CC02308G/cit15/1
  publication-title: J. Am. Chem. Soc.
  doi: 10.1021/ja305734x
– volume: 112
  start-page: 9608
  year: 2015
  ident: D3CC02308G/cit24/1
  publication-title: Proc. Natl. Acad. Sci. U. S. A.
  doi: 10.1073/pnas.1422605112
– volume: 355
  start-page: 564
  year: 1992
  ident: D3CC02308G/cit25/1
  publication-title: Nature
  doi: 10.1038/355564a0
– volume: 11
  start-page: 80
  year: 2018
  ident: D3CC02308G/cit34/1
  publication-title: Pharmaceuticals
  doi: 10.3390/ph11030080
– volume: 417
  start-page: 876
  year: 2002
  ident: D3CC02308G/cit20/1
  publication-title: Nature
  doi: 10.1038/nature755
– volume: 133
  start-page: 9824
  year: 2011
  ident: D3CC02308G/cit18/1
  publication-title: J. Am. Chem. Soc.
  doi: 10.1021/ja200786q
– start-page: 4995
  year: 2019
  ident: D3CC02308G/cit38/1
  publication-title: Eur. J. Org. Chem.
  doi: 10.1002/ejoc.201900692
– volume: 25
  start-page: 417
  year: 2019
  ident: D3CC02308G/cit37/1
  publication-title: Chem. – Eur. J.
  doi: 10.1002/chem.201802691
– volume: 224
  start-page: 344
  year: 2023
  ident: D3CC02308G/cit39/1
  publication-title: Int. J. Biol. Macromol.
  doi: 10.1016/j.ijbiomac.2022.10.128
– volume: 9
  start-page: 8618
  year: 2019
  ident: D3CC02308G/cit22/1
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-019-44736-9
– volume: 47
  start-page: 5557
  year: 2008
  ident: D3CC02308G/cit43/1
  publication-title: Angew. Chem., Int. Ed.
  doi: 10.1002/anie.200801235
– volume: 268
  start-page: 17651
  year: 1993
  ident: D3CC02308G/cit31/1
  publication-title: J. Biol. Chem.
  doi: 10.1016/S0021-9258(17)46749-4
– volume: 48
  start-page: 682
  year: 2020
  ident: D3CC02308G/cit21/1
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkz1138
– volume: 13
  start-page: 515
  year: 2003
  ident: D3CC02308G/cit52/1
  publication-title: Oligonucleotides
  doi: 10.1089/154545703322860825
– volume: 1861
  start-page: 1382
  year: 2017
  ident: D3CC02308G/cit23/1
  publication-title: Biochim. Biophys. Acta
  doi: 10.1016/j.bbagen.2017.01.006
– volume: 325
  start-page: 764
  year: 2009
  ident: D3CC02308G/cit8/1
  publication-title: Science
  doi: 10.1126/science.1175653
– volume: 17
  start-page: 1703
  year: 2022
  ident: D3CC02308G/cit40/1
  publication-title: ACS Chem. Biol.
  doi: 10.1021/acschembio.1c00904
– volume: 13
  start-page: 8371
  year: 2022
  ident: D3CC02308G/cit54/1
  publication-title: Chem. Sci.
  doi: 10.1039/D2SC01196D
– volume: 111
  start-page: 3089
  year: 2020
  ident: D3CC02308G/cit6/1
  publication-title: Cancer Sci.
  doi: 10.1111/cas.14540
– volume: 217
  start-page: 107649
  year: 2021
  ident: D3CC02308G/cit30/1
  publication-title: Pharmacol. Ther.
  doi: 10.1016/j.pharmthera.2020.107649
– volume: 42
  start-page: 11612
  year: 2014
  ident: D3CC02308G/cit42/1
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gku833
– volume: 123
  start-page: 10833
  year: 2011
  ident: D3CC02308G/cit48/1
  publication-title: Angew. Chem., Int. Ed.
  doi: 10.1002/ange.201105193
– volume: 111
  start-page: 2990
  year: 2014
  ident: D3CC02308G/cit16/1
  publication-title: Proc. Natl. Acad. Sci. U. S. A.
  doi: 10.1073/pnas.1321436111
– volume: 90
  start-page: 3745
  year: 1993
  ident: D3CC02308G/cit28/1
  publication-title: Proc. Natl. Acad. Sci. U. S. A.
  doi: 10.1073/pnas.90.8.3745
– volume: 13
  start-page: 539
  year: 2013
  ident: D3CC02308G/cit44/1
  publication-title: Chem. Rec.
  doi: 10.1002/tcr.201300015
– volume: 33
  start-page: e138
  year: 2005
  ident: D3CC02308G/cit47/1
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gni134
– volume: 34
  start-page: 2723
  year: 2006
  ident: D3CC02308G/cit19/1
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkl348
– volume: 284
  start-page: 23622
  year: 2009
  ident: D3CC02308G/cit11/1
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M109.018028
– volume: 40
  start-page: 8119
  year: 2012
  ident: D3CC02308G/cit29/1
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gks512
– volume: 35
  start-page: 406
  year: 2007
  ident: D3CC02308G/cit5/1
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkl1057
– volume: 25
  start-page: 3686
  year: 2020
  ident: D3CC02308G/cit7/1
  publication-title: Molecules
  doi: 10.3390/molecules25163686
– volume: 43
  start-page: 668
  year: 2004
  ident: D3CC02308G/cit1/1
  publication-title: Angew. Chem., Int. Ed.
  doi: 10.1002/anie.200300589
– volume: 24
  start-page: 6238
  year: 2022
  ident: D3CC02308G/cit41/1
  publication-title: Phys. Chem. Chem. Phys.
  doi: 10.1039/D1CP04718C
– volume: 370
  start-page: 468
  year: 1981
  ident: D3CC02308G/cit26/1
  publication-title: Ann. N. Y. Acad. Sci.
  doi: 10.1111/j.1749-6632.1981.tb29757.x
– volume: 28
  start-page: 1969
  year: 2000
  ident: D3CC02308G/cit53/1
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/28.9.1969
– volume: 42
  start-page: 183
  year: 2007
  ident: D3CC02308G/cit49/1
  publication-title: Methods
  doi: 10.1016/j.ymeth.2006.10.004
– volume: 115
  start-page: 194
  year: 2015
  ident: D3CC02308G/cit50/1
  publication-title: Biochimie
  doi: 10.1016/j.biochi.2015.06.002
– volume: 128
  start-page: 5966
  year: 2006
  ident: D3CC02308G/cit46/1
  publication-title: J. Am. Chem. Soc.
  doi: 10.1021/ja0603958
– volume: 10
  start-page: 43319
  year: 2020
  ident: D3CC02308G/cit45/1
  publication-title: RSC Adv.
  doi: 10.1039/D0RA09413G
– volume: 285
  start-page: 22003
  year: 2010
  ident: D3CC02308G/cit13/1
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M110.101923
– volume: 99
  start-page: 11593
  year: 2002
  ident: D3CC02308G/cit10/1
  publication-title: Proc. Natl. Acad. Sci. U. S. A.
  doi: 10.1073/pnas.182256799
– volume: 1
  start-page: 263
  year: 1993
  ident: D3CC02308G/cit17/1
  publication-title: Structure
  doi: 10.1016/0969-2126(93)90015-9
– volume: 485
  start-page: 228
  year: 1986
  ident: D3CC02308G/cit27/1
  publication-title: Ann. N. Y. Acad. Sci.
  doi: 10.1111/j.1749-6632.1986.tb34585.x
– volume: 39
  start-page: 1343
  year: 2010
  ident: D3CC02308G/cit14/1
  publication-title: Eur. Biophys. J.
  doi: 10.1007/s00249-010-0587-x
– volume: 57
  start-page: 7236
  year: 2021
  ident: D3CC02308G/cit55/1
  publication-title: Chem. Commun.
  doi: 10.1039/D1CC03294A
SSID ssj0000158
Score 2.424654
Snippet Thrombin-binding aptamer (TBA), which forms a G-quadruplex (G4) structure with anti-parallel topology, interacts with thrombin to inhibit its enzymatic...
SourceID proquest
pubmed
crossref
rsc
SourceType Aggregation Database
Index Database
Publisher
StartPage 8862
SubjectTerms Binding
Ligands
Thrombin
Topology
Title Regulation of thrombin activity by ligand-induced topological alteration in a thrombin-binding aptamer
URI https://www.ncbi.nlm.nih.gov/pubmed/37377065
https://www.proquest.com/docview/2836347333
https://www.proquest.com/docview/2830672097
Volume 59
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Zb9NAEF6F9AFeEFchUNAieHWxvV4fj1VIKRCKRB0pb9aeIUprV4ktRP8Uf5G9fBQqBLxY9sRHtPN5d2Y88w0ArwWOkfAD6mHJqRexCHvEZ9yTWcgDymlGDMXGp9P4ZBF9WOLlaPRjkLXU1PSQXd1YV_I_WlUypVddJfsPmu1uqgRqX-lXbZWG1favdPzFNpJ3Np_ueHChHF3Dj2FaQijT8ny9IiX3lOfd6C_9te2JYCkCzg2jskt2JN312lk2pS7ksiYXLnu3JTNo-QXYsLDERG676i8zvdreIIM4wxnZEdsi--xrtar6SLhZBJoeX-tttSHfjDgnG1LvmlXTAXgjaLNxtTkfyVVTVtv1MHARIh0RtXWnLuNJh0fa3FSTe-I63A2mY4QzL0GWcPJQOFkcecrPWg7ncMcqbrFqGa_djJymbrYX7SG-ceXwkSZe5Ygx7ZWlq359bHMCTj8Xx4v5vMhny_wW2AuVXxKOwd7RLH8_HzCWmZaw3R9vGXFR9qa_93Ub6DfHRpk527b9jDFz8nvgrvNP4JEF230wEuUDcLsbtIdA9qCDlYQtaGALOki_w-uggwPQwR50UF8EfwUddKB7BBbHs3x64rlmHR5TNlDtSUoDjlIeSM5QgmWEuAxZ6suQSixiGUtBkDqHKo8iYVTZkSknIiaCizQioUD7YFxWpXgCIA5ElOAQ4SCRUSBoighGarFJ_YSzIAwn4FU7fMWl5WQpTC4Fyoq3aDo1g_xuAg7akS3cO7srlDEdoyhBCE3Ay-5nNYL6MxkpRdWYc3R-gp8lE_DYaqR7DEpQohMDJmBfqagT96p9-uenPgN3-lfhAIzrbSOeK6u2pi8ckn4Cs4qqIQ
linkProvider Royal Society of Chemistry
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Regulation+of+thrombin+activity+by+ligand-induced+topological+alteration+in+a+thrombin-binding+aptamer&rft.jtitle=Chemical+communications+%28Cambridge%2C+England%29&rft.au=Sasaki%2C+Shogo&rft.au=Ma%2C+Yue&rft.au=Hirokawa%2C+Takatsugu&rft.au=Ikebukuro%2C+Kazunori&rft.date=2023-07-13&rft.pub=Royal+Society+of+Chemistry&rft.issn=1359-7345&rft.eissn=1364-548X&rft.volume=59&rft.issue=57&rft.spage=8862&rft.epage=8865&rft_id=info:doi/10.1039%2Fd3cc02308g&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1359-7345&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1359-7345&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1359-7345&client=summon