The relevance of DHA with modulating of host-gut microbiome signatures alterations and repairing of lipids metabolism shifts

• Published in: European journal of pharmacology Vol. 895; p. 173885
• Main Authors: Yu, Haining, Fang, Chengjie, Li, Peng, Wu, Manman, Shen, Shengrong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.03.2021
• Subjects: Animals; Anti-Bacterial Agents - toxicity; Bacteria - drug effects; Bacteria - growth & development; Bacteria - metabolism; Bile acids; Bile Acids and Salts - metabolism; Biomarkers - blood; DHA; Docosahexaenoic Acids - metabolism; Docosahexaenoic Acids - pharmacology; Dysbiosis; Fatty acids; Fatty Acids - blood; Feces - chemistry; Female; Gastrointestinal Microbiome - drug effects; Gut microbiome; High-Throughput Screening Assays; Intestines - microbiology; Lipid Metabolism - drug effects; Lipidomics; Lipids metabolism; Liver - drug effects; Liver - metabolism; Male; Metabolome - drug effects; Mice; Mice, Inbred BALB C; Lipids metabolism; Bile acids; DHA; Gut microbiome; Fatty acids
• DOI: 10.1016/j.ejphar.2021.173885
• ISSN: 0014-2999; 1879-0712

Huge of previous reports recommended that gut microbiome have a crucial role in the human health and its change was profound impact for the metabolic improvements associated with lipids metabolism. In order to explore the relevance of a direct dysbiosis effect of gut microbiome on lipids metabolism shifts and repaired position of DHA, we built the animal model for the study with gut microbiome dysbiosis administrated by i.g. with CRO and intervened by DHA in the present work. Gut microbiome was analyzed by high throughput sequencing and bioinformatics analyses of bacteria. The composition of fatty acids and short chain fatty acids (SCFAs) were determined by gas chromatography. Blood lipids and bile acids were assayed by kit and UPLC-MS/MS, respectively. The expressions of enzymes of long chain fatty acid metabolism were analyzed by qRT-PCR. The results showed that gut microbiome dysbiosis caused lipid metabolism abnormal, and DHA was able to repair the lipids metabolism shifts resulted from gut microbiome dysbiosis. DHA could modulate host-gut microbiome signatures, improve concentrations of SCFAs, regulate fatty acids metabolism but modify bile acid profiles. In conclusion, we considered that DHA repaired lipid metabolism by modulating gut microbiome and regulating fatty acids metabolism pathway.