Biochars assisted phytoremediation of polycyclic aromatic hydrocarbons contaminated agricultural soil: Dynamic responses of functional genes and microbial community

• Published in: Environmental pollution (1987) Vol. 345; p. 123476
• Main Authors: Guo, Meixia, Shang, Xingtian, Ma, Yulong, Zhang, Keke, Zhang, Ling, Zhou, Yanmei, Gong, Zongqiang, Miao, Renhui
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.03.2024
• Subjects: Bacteria - genetics; Bacteria - metabolism; Bacterial activity; Biochar; Biodegradation, Environmental; Charcoal; Fungal community structure; Intensified phytoremediation; Microbiota; PAHs; Polycyclic Aromatic Hydrocarbons - analysis; Soil - chemistry; Soil Microbiology; Soil Pollutants - analysis; PAHs; Fungal community structure; Bacterial activity; Intensified phytoremediation; Biochar
• DOI: 10.1016/j.envpol.2024.123476
• ISSN: 0269-7491

A biochar-intensified phytoremediation experiment was designed to investigate the dynamic effects of different biochars on polycyclic aromatic hydrocarbon (PAH) removal in ryegrass rhizosphere contaminated soil. Maize and wheat straw biochar pyrolyzed at 300 °C and 500 °C were amended into PAH-contaminated soil, and then ryegrass (Lolium multiflorum L.) was planted for 90 days. Spearman's correlations among PAH removal, enzyme activity, abundance of PAH-ring hydroxylating dioxygenase (PAH-RHDα), and fungal and bacterial community structure were analyzed to elucidate the microbial degradation mechanisms during the combined remediation process. The results showed that 500 °C wheat straw biochar had higher surface area and more nutrients, and significantly accelerated the phytoremediation of PAHs (62.5 %), especially for high molecular weight PAH in contaminated soil. The activities of urease and dehydrogenase and the abundance of total and PAH-degrading bacteria, which improved with time by biochar and ryegrass, had a positive correlation with the removal rate of PAHs. Biochar enhanced the abundance of gram-negative (GN) PAH-RHDα genes. The GN PAH-degraders, Sphingomonas, bacteriap25, Haliangium, and Dongia may play vital roles in PAH degradation in biochar-amended rhizosphere soils. Principal coordinate analysis indicated that biochar led to significant differences in fungal community structures before 30 days, while the diversity of the bacterial community composition depended on planting ryegrass after 60 days. These findings imply that the structural reshaping of microbial communities results from incubation time and the selection of biochar and ryegrass in PAH-contaminated soils. Applying 500 °C wheat straw biochar could enhance the rhizoremediation of PAH-contaminated soil and benefit the soil microbial ecology. [Display omitted] •500 °C wheat straw biochar enhanced the phytoremediation of PAH-contaminated soil.•A mechanism for PAH removal in the combined remediation has been revealed.•The synergistic effect of straw biochar and plant enhanced the growth of degraders.•Biochar and ryegrass stimulated the bacterial activity in early PAH degradation.•Biochar selectively improved the growth of bacteria and fungi in rhizosphere soil.