Expression of Id2 in the developing limb is associated with zones of active BMP signaling and marks the regions of growth and differentiation of the developing digits

Here we report the pattern of expression of inhibitor of DNA binding/differentiation factor 2 (Id2) in the developing chicken limb. We show that prior to stage 25, Id2 is expressed in the anterior and posterior mesoderm, the AER, and in the early skeletal chondrogenic aggregates. At more advanced st...

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Published inThe International journal of developmental biology Vol. 53; no. 8-10; pp. 1495 - 1502
Main Authors Lorda-Diez, Carlos I, Torre-Pérez, Nuria, García-Porrero, Juan A, Hurle, Juan M, Montero, Juan A
Format Journal Article
LanguageEnglish
Published Spain 2009
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Abstract Here we report the pattern of expression of inhibitor of DNA binding/differentiation factor 2 (Id2) in the developing chicken limb. We show that prior to stage 25, Id2 is expressed in the anterior and posterior mesoderm, the AER, and in the early skeletal chondrogenic aggregates. At more advanced stages of limb development Id2 is expressed in the undifferentiated subectodermal and interdigital mesenchyme and exhibits specific domains of expression in the growing digits. These expression domains were closely coincident with zones of activation of BMP-signaling as deduced from the distribution of phosphorylated SMADs 1/5/8. In micromass cultures transcripts of Id2 are associated with the nodules of chondrogenic differentiation. Expression of Id2 both in vivo and in vitro was up-regulated in experiments of BMP-gain-of-function and down-regulated after treatments with BMP-antagonists. Interestingly, interdigital application of TGFbeta2 transiently upregulates Id2 in coincidence with the inhibition of interdigital cell death and the commitment of the interdigital mesenchyme to form an ectopic digit. These data suggest that Id2 is a molecular mediator of BMP signaling acting in concert with the TGFbeta pathway during the formation of the digits.
AbstractList Here we report the pattern of expression of inhibitor of DNA binding/differentiation factor 2 (Id2) in the developing chicken limb. We show that prior to stage 25, Id2 is expressed in the anterior and posterior mesoderm, the AER, and in the early skeletal chondrogenic aggregates. At more advanced stages of limb development Id2 is expressed in the undifferentiated subectodermal and interdigital mesenchyme and exhibits specific domains of expression in the growing digits. These expression domains were closely coincident with zones of activation of BMP-signaling as deduced from the distribution of phosphorylated SMADs 1/5/8. In micromass cultures transcripts of Id2 are associated with the nodules of chondrogenic differentiation. Expression of Id2 both in vivo and in vitro was up-regulated in experiments of BMP-gain-of-function and down-regulated after treatments with BMP-antagonists. Interestingly, interdigital application of TGFbeta2 transiently upregulates Id2 in coincidence with the inhibition of interdigital cell death and the commitment of the interdigital mesenchyme to form an ectopic digit. These data suggest that Id2 is a molecular mediator of BMP signaling acting in concert with the TGFbeta pathway during the formation of the digits.
Here we report the pattern of expression of inhibitor of DNA binding/differentiation factor 2 (Id2) in the developing chicken limb. We show that prior to stage 25, Id2 is expressed in the anterior and posterior mesoderm, the AER, and in the early skeletal chondrogenic aggregates. At more advanced stages of limb development Id2 is expressed in the undifferentiated subectodermal and interdigital mesenchyme and exhibits specific domains of expression in the growing digits. These expression domains were closely coincident with zones of activation of BMP-signaling as deduced from the distribution of phosphorylated SMADs 1/5/8. In micromass cultures transcripts of Id2 are associated with the nodules of chondrogenic differentiation. Expression of Id2 both in vivo and in vitro was up-regulated in experiments of BMP-gain-of-function and down-regulated after treatments with BMP-antagonists. Interestingly, interdigital application of TGFb2 transiently upregulates Id2 in coincidence with the inhibition of interdigital cell death and the commitment of the interdigital mesenchyme to form an ectopic digit. These data suggest that Id2 is a molecular mediator of BMP signaling acting in concert with the TGFb pathway during the formation of the digits.
Author Lorda-Diez, Carlos I
Torre-Pérez, Nuria
García-Porrero, Juan A
Hurle, Juan M
Montero, Juan A
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Snippet Here we report the pattern of expression of inhibitor of DNA binding/differentiation factor 2 (Id2) in the developing chicken limb. We show that prior to stage...
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StartPage 1495
SubjectTerms Animals
Body Patterning - genetics
Body Patterning - physiology
Bone Morphogenetic Protein 2 - genetics
Bone Morphogenetic Protein 2 - metabolism
Bone Morphogenetic Protein 7 - genetics
Bone Morphogenetic Protein 7 - metabolism
Bone Morphogenetic Proteins - genetics
Bone Morphogenetic Proteins - metabolism
Bone Morphogenetic Proteins - pharmacology
Cartilage - cytology
Cartilage - metabolism
Cell Differentiation - drug effects
Cell Differentiation - genetics
Cell Differentiation - physiology
Cell Proliferation - drug effects
Cells, Cultured
Chick Embryo
Chondrogenesis - drug effects
Extremities - embryology
Gene Expression Regulation, Developmental - drug effects
Immunohistochemistry
In Situ Hybridization
Inhibitor of Differentiation Protein 2 - genetics
Inhibitor of Differentiation Protein 2 - metabolism
Mesoderm - cytology
Mesoderm - metabolism
Microscopy, Confocal
Phosphorylation
Signal Transduction - genetics
Signal Transduction - physiology
Smad1 Protein - metabolism
Smad5 Protein - metabolism
Smad8 Protein - metabolism
Transforming Growth Factor beta - pharmacology
Title Expression of Id2 in the developing limb is associated with zones of active BMP signaling and marks the regions of growth and differentiation of the developing digits
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