A kinome wide screen identifies novel kinases involved in regulation of monoamine transporter function

• Published in: Neurochemistry international Vol. 98; pp. 103 - 114
• Main Authors: Vuorenpää, Anne, Ammendrup-Johnsen, Ina, Jørgensen, Trine N., Gether, Ulrik
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2016
• Subjects: Dopamine Plasma Membrane Transport Proteins - genetics; Dopamine Plasma Membrane Transport Proteins - metabolism; Gene Expression Regulation - genetics; Gene Expression Regulation - physiology; HEK293 Cells; High-Throughput Screening Assays; Humans; Kinase; Monoamine transporter; Neurotransmitter Agents - metabolism; PrKX; Protein Kinase C-alpha - genetics; Protein Kinase C-alpha - metabolism; Protein Kinases - genetics; Protein Kinases - metabolism; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; RNA - genetics; Serotonin Plasma Membrane Transport Proteins - genetics; Serotonin Plasma Membrane Transport Proteins - metabolism; siRNA screen; Surface expression; Uptake; Vesicular Monoamine Transport Proteins - genetics; Vesicular Monoamine Transport Proteins - metabolism; Vesicular Monoamine Transport Proteins - physiology; PMA; Surface expression; siRNA screen; PrKX; siRNA; SLC; EGFP; CAD cells; Uptake; SERT; β2-AR; Kinase; DAT; Monoamine transporter; NE; SIK3; NET; 5-HT; PKA C-α; DA

The high affinity transporters for the monoamine neurotransmitters, dopamine, norepinephrine, and serotonin, play a key role in controlling monoaminergic neurotransmission. It is believed that the transporters (DAT, NET and SERT, respectively) are subject to tight regulation by the cellular signalin...