Validation of an eight parameter immunophenotyping panel in adult canines for assessment of immunotoxicity

• Published in: Veterinary immunology and immunopathology Vol. 154; no. 1-2; pp. 75 - 81
• Main Authors: Zeigler, Brandon M., Boyle-Holmes, Yvonne, Falzone, Deanna, Farmer, John
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.07.2013
• Subjects: Animals; Antigens, CD - genetics; Antigens, CD - metabolism; Canine; Dogs - immunology; Dogs - metabolism; Female; Flow cytometry; Flow Cytometry - veterinary; Gene Expression Regulation - immunology; Immunotoxicology; Leukocytes; Leukocytes - metabolism; Male; Reproducibility of Results; Flow cytometry; Immunotoxicology; Leukocytes; Canine

Analysis of peripheral blood leukocyte populations by flow cytometry in adult beagles is a critical component of immunotoxicity assessment in regulated pre-clinical toxicology studies. In this study, data is presented utilizing a single panel, six-color method to simultaneously enumerate absolute ce...