Bioaccumulation and oxidative damage of polycyclic aromatic hydrocarbon mixtures in Manila clam Ruditapes philippinarum

• Published in: Ecotoxicology and environmental safety Vol. 197; p. 110558
• Main Authors: Li, Dongyu, Liu, Tong, Pan, Luqing, Hu, Fengxiao, Jin, Qian
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.07.2020
• Subjects: Bioaccumulation; Detoxification; Equal proportion; Oxidative damage; Polycyclic aromatic hydrocarbons mixture; Ruditapes philippinarum; Equal proportion; Bioaccumulation; Oxidative damage; Polycyclic aromatic hydrocarbons mixture; Ruditapes philippinarum; Detoxification
• ISSN: 0147-6513; 1090-2414; 1090-2414
• DOI: 10.1016/j.ecoenv.2020.110558

The aim of this study was to investigate the bioaccumulation and oxidative damage of Manila clam, Ruditapes philippinarum, exposed to four selected mixtures of polycyclic aromatic hydrocarbons (PAHs; benzo (a) pyrene (BaP), benzo (a) anthracene (BaA), benzo (b) fluoranthene (BbF), and chrysene (Chr) in equal proportion. For this purpose, clams were exposed to PAHs (BaP:BbF:BaA:Chr = 1:1:1:1) at different concentrations (0.05, 0.5, and 5 μg/L) for 21 days, followed by a 15-day depuration period. All four PAHs accumulated in the gill, digestive gland, adductor muscle, and soft tissue of Manila clams, and all PAH treatment groups showed clear time and dose dependence. The decreasing order of bioaccumulation for the four PAHs in the exposure experiment was Chr > BaA > BaP > BbF. Moreover, the order of PAH bioaccumulation for the four tissues during the whole experiment was digestive gland > gill > soft tissues > adductor muscles. Although the initial concentrations of the four PAHs were the same, the final accumulated contents were different. Therefore, we also determined the detoxification processes of the four PAH mixtures in gills and digestive glands. The bioaccumulation of Chr was higher than the other three PAHs, probably because clams have a lower metabolic capacity for Chr than for BaP, BbF, and BaA. Exposure to PAH mixtures can result in oxidative damage, as indicated by the fact that DNA strand breaks, lipid peroxidation (LPO), and protein carbonyl (PC) were induced significantly (P < 0.05), except in the low-dose groups of PAHs, and different trends were detected with time of exposure. According to the correlation analysis, aryl hydrocarbon hydroxylase, glutathione s-transferase, superoxide dismutase, DNA strand break, PC, and LPO in both the gill and digestive gland are potential early indicators of PAH mixtures. We investigated the accumulation rules of R. philippinarum exposed to the selected PAHs and screened the potential biomarkers. The results of our study provide important scientific information for the purpose of monitoring marine pollution. •The bioaccumulation of the mixture of BaP, BbF, BaA and Chr in equal proportion in four tissues was studied of Manila clam.•The detoxification process of the PAH mixture was determined in gills and digestive glands.•The oxidative damage of PAH mixture in high concentration group cannot be relieved entirely by antioxidant defenses.•The potential early warning indicators for PAHs pollution monitoring were selected.