Eukaryotic elongation factor 2 is involved in the anticoccidial action of diclazuril in the second-generation merozoites of Eimeria tenella

• Published in: Veterinary parasitology Vol. 276; p. 108991
• Main Authors: Zhou, Bian-hua, Jia, Liu-shu, Guo, Hong-wei, Ding, Hai-yan, Yang, Jing-yun, Wang, Hong-wei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2019
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Blotting, Western; Chickens - parasitology; Coccidiosis - drug therapy; Coccidiosis - parasitology; Coccidiosis - veterinary; Coccidiostats - pharmacology; Diclazuril; Eimeria tenella; Eimeria tenella - drug effects; Eimeria tenella - genetics; Elongation Factor 2 Kinase - genetics; Elongation Factor 2 Kinase - metabolism; Eukaryotic elongation factor 2; Female; Fluorescent Antibody Technique; Male; Merozoites - drug effects; Merozoites - genetics; Mice; Mice, Inbred BALB C; Nitriles - pharmacology; Phylogeny; Poultry Diseases - drug therapy; Poultry Diseases - parasitology; Real-Time Polymerase Chain Reaction; Second-generation merozoites; Sequence Alignment; Triazines - pharmacology; Eimeria tenella; Diclazuril; Second-generation merozoites; Eukaryotic elongation factor 2

•The full-length cDNA of EtEF2 of E. tenella were cloned and characterized.•EtEF2 displays high sequence homology with other eukaryotic organisms.•EtEF2 was characteristic with typical transaction related structure.•The localization of EtEF2 was primarily at the cytoplasm.•Diclazuril up-regulated th...